| http://purl.uniprot.org/citations/24389272 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24389272 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24389272 | http://www.w3.org/2000/01/rdf-schema#comment | "Three new cysteine-free venom peptides, which are referred to as Heterin-1, Heterin-2 and Spiniferin, respectively, were identified from the scorpion Heterometrus spinifer. Heterin-1, Heterin-2 and Spiniferin contain 43, 24 and 13 amino acid residues, respectively. Genomic analysis showed that the genomic organizations of the three peptides are consistent with those of the known Na(+), K(+) or Cl(-)-channel specific toxins from scorpions; this suggests that the genes of the cysteine-free and cysteine-rich peptides from scorpions were derived from a common ancestor. Antimicrobial assay demonstrated that Heterin-1 possesses potent activities against both Gram-positive and Gram-negative bacteria. Among the tested bacterial species, Heterin-1 is the most active against Bacillus megaterium and Micrococcus luteus with MICs of 4.0 μM and 4.0 μM, respectively. Heterin-2 is able to potently inhibit the growth of Gram-positive bacteria with MICs from 5.6 μM to 30.0 μM; however, it has weaker activities against the tested Gram-negative bacteria. It is interesting to see that deletion of the C-terminal random coiled tail (KKD) in Heterin-2 markedly changed the antimicrobial specificity and activity of the peptide. Spiniferin has very weak antimicrobial activities against both Gram-positive and Gram-negative bacteria. We found that introducing three net charges into the polar face of Spiniferin significantly increased its antimicrobial activity against the majority of the tested bacteria; however, in some instances, net charge on the polar face is not important for the antimicrobial activity of the peptide. These studies have expanded our understanding of the diversity, evolution and structure/function relationships of the cysteine-free peptides from scorpions."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.peptides.2013.12.012"xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.peptides.2013.12.012"xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Cao H."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Cao H."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Luo X."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Luo X."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Nie Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Nie Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zhou L."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zhou L."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Wu S."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Wu S."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zeng X.C."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/author | "Zeng X.C."xsd:string |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24389272 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |