| http://purl.uniprot.org/citations/2439522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2439522 | http://www.w3.org/2000/01/rdf-schema#comment | "The platelet-derived growth factor (PDGF) modulated growth response of the MG-63 human osteosarcoma cell line, which neither expresses c-sis mRNA nor secretes a PDGF analogue, was characterized. Scatchard analysis demonstrated that the MG-63 cells have 23,000 receptors per cell with a Kd of 5 X 10(-11) M. The receptor became phosphorylated, in a PDGF concentration-dependent manner, when 32P-orthophosphate-labeled cells were treated with PDGF for 3 h at 4 degrees C. The phosphorylated receptor was identified by autoradiography and gel electrophoresis after isolation of the 32P-labeled receptor using a solid-phase monoclonal antibody directed against phosphotyrosine. Binding of the receptor to the antibody was inhibited by 5 mM phenyl phosphate, further suggesting that PDGF stimulated tyrosine-specific receptor autophosphorylation. In addition, treatment of MG-63 cells with PDGF for 3 h at 37 degrees C induced a 7.5-fold increase in c-myc mRNA accumulation as analyzed on Northern gels. However, MG-63 cells grew equally well in either serum-(which contains PDGF) or plasma-(which does not) supplemented medium. Furthermore, PDGF did not stimulate DNA synthesis in growth arrested MG-63 cells, nor did it potentiate DNA synthesis modulated by somatomedin C. Thus MG-63 cells are a naturally occurring cell variant in which PDGF stimulates c-myc expression but does not modulate mitogenesis."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.1041320109"xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Ross A.H."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Mitchell C.D."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Frick K."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Bishayee S."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Scher C.D."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/author | "Womer R.B."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/pages | "65-72"xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/title | "PDGF induces c-myc mRNA expression in MG-63 human osteosarcoma cells but does not stimulate cell replication."xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://purl.uniprot.org/core/volume | "132"xsd:string |
| http://purl.uniprot.org/citations/2439522 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2439522 |
| http://purl.uniprot.org/citations/2439522 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2439522 |
| http://purl.uniprot.org/uniprot/P01127#attribution-3D99F95FBADC1E30B7D23AFBE30D7535 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2439522 |
| http://purl.uniprot.org/uniprot/P04085#attribution-3D99F95FBADC1E30B7D23AFBE30D7535 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/2439522 |