| http://purl.uniprot.org/citations/24449906 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24449906 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24449906 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA polymerase ζ (Pol ζ) is a eukaryotic B-family DNA polymerase that specializes in translesion synthesis and is essential for normal embryogenesis. At a minimum, Pol ζ consists of a catalytic subunit Rev3 and an accessory subunit Rev7. Mammalian Rev3 contains >3,000 residues and is twice as large as the yeast homolog. To date, no vertebrate Pol ζ has been purified for biochemical characterization. Here we report purification of a series of human Rev3 deletion constructs expressed in HEK293 cells and identification of a minimally catalytically active human Pol ζ variant. With a tagged form of an active Pol ζ variant, we isolated two additional accessory subunits of human Pol ζ, PolD2 and PolD3. The purified four-subunit Pol ζ4 (Rev3-Rev7-PolD2-PolD3) is much more efficient and more processive at bypassing a 1,2-intrastrand d(GpG)-cisplatin cross-link than the two-subunit Pol ζ2 (Rev3-Rev7). We show that complete bypass of cisplatin lesions requires Pol η to insert dCTP opposite the 3' guanine and Pol ζ4 to extend the primers."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1324001111"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1324001111"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Lee Y.S."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Lee Y.S."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Yang W."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Gregory M.T."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/author | "Gregory M.T."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/pages | "2954-2959"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/pages | "2954-2959"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/title | "Human Pol zeta purified with accessory subunits is active in translesion DNA synthesis and complements Pol eta in cisplatin bypass."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/title | "Human Pol zeta purified with accessory subunits is active in translesion DNA synthesis and complements Pol eta in cisplatin bypass."xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/volume | "111"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://purl.uniprot.org/core/volume | "111"xsd:string |
| http://purl.uniprot.org/citations/24449906 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24449906 |
| http://purl.uniprot.org/citations/24449906 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24449906 |
| http://purl.uniprot.org/citations/24449906 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24449906 |
| http://purl.uniprot.org/citations/24449906 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24449906 |