| http://purl.uniprot.org/citations/24464102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24464102 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24464102 | http://www.w3.org/2000/01/rdf-schema#comment | "The phytochelatin synthase from photosynthetic Euglena gracilis (EgPCS) was analyzed at the transcriptional, kinetic, functional, and phylogenetic levels. Recombinant EgPCS was a monomeric enzyme able to synthesize, in the presence of Zn(2+) or Cd(2+), phytochelatin2-phytochelatin4 (PC2-PC4) using GSH or S-methyl-GS (S-methyl-glutathione), but not γ-glutamylcysteine or PC2 as a substrate. Kinetic analysis of EgPCS firmly established a two-substrate reaction mechanism for PC2 synthesis with Km values of 14-22 mM for GSH and 1.6-2.5 μM for metal-bis-glutathionate (Me-GS2). EgPCS showed the highest Vmax and catalytic efficiency with Zn-(GS)2, and was inactivated by peroxides. The EgPCS N-terminal domain showed high similarity to that of other PCSases, in which the typical catalytic core (Cys-70, His-179 and Asp-197) was identified. In contrast, the C-terminal domain showed no similarity to other PCSases. An EgPCS mutant comprising only the N-terminal 235 amino acid residues was inactive, suggesting that the C-terminal domain is essential for activity/stability. EgPCS transcription in Euglena cells was not modified by Cd(2+), whereas its heterologous expression in ycf-1 yeast cells provided resistance to Cd(2+) stress. Phylogenetic analysis of the N-terminal domain showed that EgPCS is distant from plants and other photosynthetic organisms, suggesting that it evolved independently. Although EgPCS showed typical features of PCSases (constitutive expression; conserved N-terminal domain; kinetic mechanism), it also exhibited distinct characteristics such as preference for Zn-(GS)2 over Cd-(GS)2 as a co-substrate, a monomeric structure, and ability to solely synthesize short-chain PCs, which may be involved in conferring enhanced heavy-metal resistance."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.org/dc/terms/identifier | "doi:10.1039/c3mt00313b"xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.org/dc/terms/identifier | "doi:10.1039/c3mt00313b"xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Encalada R."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Encalada R."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Moreno-Sanchez R."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Moreno-Sanchez R."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Saavedra E."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Saavedra E."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Girard L."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Girard L."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Rodriguez-Zavala J.S."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Rodriguez-Zavala J.S."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Pardo J.P."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Pardo J.P."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Hernandez G."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Hernandez G."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Garcia-Garcia J.D."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Garcia-Garcia J.D."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Mendoza-Cozatl D.G."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Mendoza-Cozatl D.G."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Reyes-Prieto A."xsd:string |
| http://purl.uniprot.org/citations/24464102 | http://purl.uniprot.org/core/author | "Reyes-Prieto A."xsd:string |