| http://purl.uniprot.org/citations/24465689 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24465689 | http://www.w3.org/2000/01/rdf-schema#comment | "The transcription factor NF-κB plays a key regulatory role in lymphocyte activation and generation of immune response. Stimulation of T cell receptor (TCR) induces phosphorylation of CARMA1 by PKCθ, resulting in formation of CARMA1-Bcl10-MALT1 (CBM) complex at lipid rafts and subsequently leading to NF-κB activation. While many molecular events leading to NF-κB activation have been reported, it is less understood how this activation is negatively regulated. We performed a cell-based screening for negative regulators of TCR-mediated NF-κB activation, using mutagenesis and complementation cloning strategies. Here we show that casein kinase-2 interacting protein-1 (CKIP-1) suppresses PKCθ-CBM-NF-κB signaling. We found that CKIP-1 interacts with CARMA1 and competes with PKCθ for association. We further confirmed that a PH domain of CKIP-1 is required for association with CARMA1 and its inhibitory effect. CKIP-1 represses NF-κB activity in unstimulated cells, and inhibits NF-κB activation induced by stimulation with PMA or constitutively active PKCθ, but not by stimulation with TNFα. Interestingly, CKIP-1 does not inhibit NF-κB activation induced by CD3/CD28 costimulation, which caused dissociation of CKIP-1 from lipid rafts. These data suggest that CKIP-1 contributes maintenance of a resting state on NF-κB activity or prevents T cells from being activated by inadequate signaling. In conclusion, we demonstrate that CKIP-1 interacts with CARMA1 and has an inhibitory effect on PKCθ-CBM-NF-κB signaling."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0085762"xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Kobayashi M."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Arai Y."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Koyanagi Y."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Shindo K."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Nagata K."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Yamashita K."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Shinohara M."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Io K."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Takaori-Kondo A."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Tada K."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Sakamoto T."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Kadowaki N."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Takiuchi Y."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/author | "Iwai F."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/pages | "e85762"xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/title | "CKIP-1 is an intrinsic negative regulator of T-cell activation through an interaction with CARMA1."xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/24465689 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24465689 |
| http://purl.uniprot.org/citations/24465689 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24465689 |
| http://purl.uniprot.org/uniprot/#_A0A192GQE0-mappedCitation-24465689 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24465689 |