| http://purl.uniprot.org/citations/24489448 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24489448 | http://www.w3.org/2000/01/rdf-schema#comment | "Activation of macrophages with lipopolysaccharide (LPS) involves a sequential engagement of serum LPS-binding protein (LBP), plasma membrane CD14, and TLR4/MD-2 signaling complex. We analyzed participation of CD14 in TNF-α production stimulated with 1-1000 ng/mL of smooth or rough LPS (sLPS or rLPS) and in sLPS binding to RAW264 and J744 cells. CD14 was indispensable for TNF-α generation induced by a low concentration, 1 ng/mL, of sLPS and rLPS. At higher doses of both LPS forms (100-1000 ng/mL), TNF-α release required CD14 to much lower extent. Among the two forms of LPS, rLPS-induced TNF-α production was less CD14-dependent and could proceed in the absence of serum as an LBP source. On the other hand, the involvement of CD14 was crucial for the binding of 1000 ng/mL of sLPS judging from an inhibitory effect of the anti-CD14 antibody. The binding of sLPS was also strongly inhibited by dextran sulfate, a competitive ligand of scavenger receptors (SR). In the presence of dextran sulfate, sLPS-induced production of TNF-α was upregulated about 1.6-fold. The data indicate that CD14 together with SR participates in the binding of high doses of sLPS. However, CD14 contribution to TNF α production induced by high concentrations of sLPS and rLPS can be limited."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.org/dc/terms/identifier | "doi:10.1155/2013/824919"xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/author | "Kwiatkowska K."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/author | "Plociennikowska A."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/author | "Sobota A."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/author | "Borzecka K."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/author | "Bjorkelund H."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/name | "Mediators Inflamm"xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/pages | "824919"xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/title | "CD14 mediates binding of high doses of LPS but is dispensable for TNF-alpha production."xsd:string |
| http://purl.uniprot.org/citations/24489448 | http://purl.uniprot.org/core/volume | "2013"xsd:string |
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