| http://purl.uniprot.org/citations/24533711 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24533711 | http://www.w3.org/2000/01/rdf-schema#comment | "AimHLA-B*57:01 status needs to be determined before initiating abacavir therapy. We developed a pharmacogenetic real-time (Q)-PCR screening test using two sets of sequence specific primers. This test has been implemented into routine clinical practice.Materials & methodsHIV-infected patients admitted at our University Hospital were thus genotyped using the above mentioned test. A panel of 80 DNA samples with a known genotype were used to characterize Q-PCR conditions using different master mixes.ResultsA total of 353 patients were genotyped, detecting 15 (4.25%) HLA-B*57:01 positive carriers. Among the negative patients, 17.2% were treated with abacavir without any hypersensitivity reaction. Using different Q-PCR master mixes, significantly lower cutoff Ct values were found, thus new analytical settings are provided.ConclusionThe pharmacogenetic test developed in our laboratory for the fast screening of HLA-B*57:01 can be successfully implemented into routine clinical practice. All 16 sequences (including an additional six) currently known for the HLA-B*57:01 allele are detected by sequence specific primers used in this test. The Brilliant II SYBR(®) Green QPCR MM (Stratagene) can safely replace the master mix originally used to develop the test."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.org/dc/terms/identifier | "doi:10.2217/pgs.13.242"xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Gagliardi D."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Cauda R."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Fabbiani M."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Di Giambenedetto S."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Navarra P."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Dello Russo C."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Lisi L."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/author | "Fanti I."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/name | "Pharmacogenomics"xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/pages | "319-327"xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/title | "Detection of HLA-B*57:01 by real-time PCR: implementation into routine clinical practice and additional validation data."xsd:string |
| http://purl.uniprot.org/citations/24533711 | http://purl.uniprot.org/core/volume | "15"xsd:string |
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| http://purl.uniprot.org/uniprot/#_A0A068B107-mappedCitation-24533711 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24533711 |
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