| http://purl.uniprot.org/citations/24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24553346 | http://www.w3.org/2000/01/rdf-schema#comment | "Techniques enabling precise genome modifications enhance the safety of gene-based therapy. DLC1 is a hot spot for phiC31 integrase-mediated transgene integration in vitro and in vivo. Here we show that integration of a coagulation factor VIII transgene into intron 7 of DLC1 supports durable expression of factor VIII in primary human umbilical cord-lining epithelial cells. Oligoclonal cells with factor VIII transgene integrated in DLC1 did not have altered expression of DLC1 or neighbouring genes within a 1-Mb interval. Only 1.9% of all expressed genes were transcriptionally altered; most were downregulated and mapped to cell cycle and DNA repair pathways. DLC1-integrated cells were not tumourigenic in vivo and were normal by high-resolution genomic DNA copy number analysis. Our data identify DLC1 as a locus for durable transgene expression that does not incur features of insertional oncogenesis, thus expanding options for developing ex vivo cell therapy mediated by site-specific integration methods."xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.org/dc/terms/identifier | "doi:10.1038/gt.2014.11"xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/author | "Phan T.T."xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/author | "Kon O.L."xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/author | "Sivalingam J."xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/name | "Gene Ther"xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/pages | "402-412"xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/title | "Intragenic integration in DLC1 sustains factor VIII expression in primary human cells without insertional oncogenicity."xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/24553346 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24553346 |
| http://purl.uniprot.org/citations/24553346 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A1Y1BZT1-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A2G2-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A2G3-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A2G4-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
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| http://purl.uniprot.org/uniprot/#_A0A2G9-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A127R638-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
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| http://purl.uniprot.org/uniprot/#_A8K119-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |
| http://purl.uniprot.org/uniprot/#_A0A384MTV1-mappedCitation-24553346 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24553346 |