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http://purl.uniprot.org/citations/24585571http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24585571http://www.w3.org/2000/01/rdf-schema#comment"The chromatin remodeling complex SWI/SNF and the transcription factor C/EBPβ play critical roles in osteoblastic cells as they jointly control transcription of a number of bone-related target genes. The largest C/EBPβ isoform, LAP*, possesses a short additional N-terminal domain that has been proposed to mediate the interaction of this factor with SWI/SNF in myeloid cells. Here we examine the requirement of a functional N-terminus in C/EBPβ-LAP* for binding SWI/SNF and for recruiting this complex to the Ric-8B gene to mediate transcriptional repression. We find that both C/EBPβ-LAP* and SWI/SNF simultaneously bind to the Ric-8B promoter in differentiating osteoblasts that repress Ric-8B expression. This decreased expression of Ric-8B is not accompanied by significant changes in histone acetylation at the Ric-8B gene promoter sequence. A single aminoacid change at the C/EBPβ-LAP* N-terminus (R3L) that inhibits C/EBPβ-LAP*-SWI/SNF interaction, also prevents SWI/SNF recruitment to the Ric-8B promoter as well as C/EBPβ-LAP*-dependent repression of the Ric-8B gene. Inducible expression of the C/EBPβ-LAP*R3L protein in stably transfected osteoblastic cells demonstrates that this mutant protein binds to C/EBPβ-LAP*-target promoters and competes with the endogenous C/EBPβ factor. Together our results indicate that a functional N-terminus in C/EBPβ-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.org/dc/terms/identifier"doi:10.1002/jcp.24595"xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Stein G.S."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Stein J.L."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"van Wijnen A.J."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Lian J.B."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Montecino M."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Meza D."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Aguilar R."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Grandy R."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Sepulveda H."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/author"Pihan P."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/name"J Cell Physiol"xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/pages"1521-1528"xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/title"A functional N-terminal domain in C/EBPbeta-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts."xsd:string
http://purl.uniprot.org/citations/24585571http://purl.uniprot.org/core/volume"229"xsd:string
http://purl.uniprot.org/citations/24585571http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24585571
http://purl.uniprot.org/citations/24585571http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/24585571
http://purl.uniprot.org/uniprot/#_Q80XE1-mappedCitation-24585571http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24585571
http://purl.uniprot.org/uniprot/Q80XE1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/24585571