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http://purl.uniprot.org/citations/24594606http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24594606http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24594606http://www.w3.org/2000/01/rdf-schema#comment"

Background

The gene for New Delhi metallo-β-lactamase 1 (NDM-1) has been reported to be transmitted via plasmids which are easily transferable and capable of wide distribution. We report the isolation of two NDM-1 producing strains and possible in vivo transfer of blaNDM-1 in a patient.

Methods

Clinical samples were collected for bacterial culture and antibiotic susceptibility testing from a patient during a 34-day hospitalization. The presence of blaNDM-1 was detected by PCR and sequencing. Plasmids of interest were sequenced. Medical records were reviewed for evidence of association between the administration of antibiotics and the acquisition of the NDM-1 resistance.

Results

A NDM-1 positive Raoultella planticola was isolated from blood on the ninth day of hospitalization without administration of any carbapenem antibiotics and a NDM-1 positive Escherichia coli was isolated from feces on the 29th day of hospitalization and eight days after imipenem administration. The blaNDM-1 was carried by a 280 kb plasmid pRpNDM1-1 in R. planticola and a 58 kb plasmid pEcNDM1-4 in E. coli. The two plasmids shared a 4812 bp NDM-1-ISCR1 element which was found to be excisable from the plasmid as a free form and transferrable in vitro to a NDM-1 negative plasmid from E. coli.

Conclusion

blaNDM-1 was embedded in an ISCR1 complex class 1 integron as a novel 4812 bp NDM-1-ISCR1 element. The element was found to be able to self excise to become a free form, which may provide a new vehicle for NDM-1 dissemination. This mechanism could greatly accelerate the spread of NDM-1 mediated broad spectrum β-lactam resistance."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0089893"xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Chen X."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Chen X."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Chen Q."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Chen Q."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Liu B."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Liu B."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Lin W."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Lin W."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Lan R."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Lan R."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Zhao H."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Zhao H."xsd:string
http://purl.uniprot.org/citations/24594606http://purl.uniprot.org/core/author"Xu J."xsd:string
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