http://purl.uniprot.org/citations/24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24602872 | http://www.w3.org/2000/01/rdf-schema#comment | "The 50-amino acid protein medin is the main fibrillar component of human aortic medial amyloid (AMA), the most common form of localised amyloid which affects 97% of Caucasians over the age of 50. Structural models for several amyloid assemblies, including the Alzheimer's amyloid-β peptides, have been defined from solid-state nuclear magnetic resonance (SSNMR) measurements on (13)C- and (15)N-labelled protein fibrils. SSNMR-derived structural information on fibrillar medin is scant, however, because studies to date have been restricted to limited measurements on site-specifically labelled protein prepared by solid-phase synthesis. Here we report a procedure for the expression of a SUMO-medin fusion protein in Escherichia coli and IMAC purification yielding pure, uniformly (13)C,(15)N-labelled medin in quantities required for SSNMR analysis. Thioflavin T fluorescence and dynamic light scattering measurements and transmission electron microscopy analysis confirm that recombinant medin assembles into amyloid-like fibrils over a 48-h period. The first (13)C and (15)N SSNMR spectra obtained for uniformly-labelled fibrils indicate that medin adopts a predominantly β-sheet conformation with some unstructured elements, and provide the basis for further, more detailed structural investigations."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2014.02.009"xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/author | "Gibson R.P."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/author | "Wilkinson M.C."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/author | "Davies H.A."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/author | "Middleton D.A."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/name | "Protein Expr Purif"xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/pages | "32-37"xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/title | "Expression and purification of the aortic amyloid polypeptide medin."xsd:string |
http://purl.uniprot.org/citations/24602872 | http://purl.uniprot.org/core/volume | "98"xsd:string |
http://purl.uniprot.org/citations/24602872 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24602872 |
http://purl.uniprot.org/citations/24602872 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24602872 |
http://purl.uniprot.org/uniprot/#_A0A8Q3SIZ9-mappedCitation-24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/#_B4E396-mappedCitation-24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/#_B3KTQ2-mappedCitation-24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/#_H0YKB5-mappedCitation-24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/#_Q08431-mappedCitation-24602872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/B4E396 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/A0A8Q3SIZ9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/Q08431 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/H0YKB5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24602872 |
http://purl.uniprot.org/uniprot/B3KTQ2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24602872 |