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http://purl.uniprot.org/citations/24612089http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24612089http://www.w3.org/2000/01/rdf-schema#comment"

Background

We recently showed that miR-494 was downregulated in gastric carcinoma (GC). The objectives of this study were to determine the role of miR-494 in GC malignancy and to identify its target genes.

Methods

Real-time polymerase chain reaction was employed to quantify the expression level of miR-494 and c-myc in gastric cancer tissues. Bioinformatics was used to predict the downstream target genes of miR-494, which were confirmed by luciferase and RNA immunoprecipitation assays. Cell functional analyses and a xenograft mouse model were used to evaluate the role of miR-494 in malignancy.

Results

miR-494 was downregulated in human GC tissues and in GC cells and was negatively correlated with c-myc expression. High level of c-myc or low level of miR-494 correlated with poor prognosis. The miR-494-binding site in the c-myc 3' untranslated region was predicted using TargetScan and was confirmed by the luciferase assay. Additionally, c-myc and miR-494 were enriched in coimmunoprecipitates with tagged Argonaute2 proteins in cells overexpressing miR-494. Furthermore, a miR-494 mimic significantly downregulated endogenous c-myc expression, which may contribute to the delayed G1/S transition, decreased synthesis phase bromodeoxyuridine incorporation, and impaired cell growth and colony formation; on the other hand, treatment with a miR-494 inhibitor displayed the opposite effects. Reduced tumor burden and decreased cell proliferation were observed following the delivery of miR-494 into xenograft mice.

Conclusion

miR-494 is downregulated in human GC and acts as an anti-oncogene by targeting c-myc. miR-494 plays a role in the pathogenesis of gastric cancer in a recessive fashion."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.org/dc/terms/identifier"doi:10.1111/jgh.12558"xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Chen X."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"He Y."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Ke Z."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Li Y."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Lu L."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Pan Y."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Tang B."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Wang Z."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"Cai S."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/author"He W."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/name"J Gastroenterol Hepatol"xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/pages"1427-1434"xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/title"miR-494 acts as an anti-oncogene in gastric carcinoma by targeting c-myc."xsd:string
http://purl.uniprot.org/citations/24612089http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/24612089http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24612089
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