| http://purl.uniprot.org/citations/24622775 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24622775 | http://www.w3.org/2000/01/rdf-schema#comment | "Fat-1 transgenic mice, which endogenously convert n-6 PUFA to n-3 PUFA, are a useful tool in health research; however with this model timing of n-3 PUFA enrichment cannot be directly controlled. To add such capability, the novel Cre-recombinase inducible fat-1 (iFat1) transgenic mouse has been developed. The aim of this study was to characterize the utility of the iFat1 transgene as a model of Cre-inducible endogenous n-3 PUFA enrichment. Functionality of the iFat1 transgene was screened both in vitro and in vivo. In the presence of Cre, the iFat1 transgene resulted in a balancing (p < 0.01) of the n-6/n-3 PUFA ratio within phospholipids in the human embryonic kidney 293T cell line. For in vivo analysis, iFat1 transgenic mice were crossed with the R26-Cre-ER(T2) (Tam-Cre) mouse line, a tamoxifen inducible Cre-expression model. Tam-Cre/iFat1 double hybrids were transiently treated with tamoxifen at 6-7 weeks, then terminated 3 weeks later. Tamoxifen treated mice had increased (p < 0.05) tissue n-3 PUFA and ≥two-fold reduction (p < 0.05) in the n-6/n-3 PUFA ratio of liver, kidney and muscle phospholipids relative to vehicle treated controls. Collectively these findings suggest that the iFat1 transgenic mouse may be a promising tool to help elucidate the temporal effects through which n-3 PUFA impacts health related outcomes."xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11248-014-9788-x"xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/author | "Clarke S.E."xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/author | "Kang J.X."xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/author | "Ma D.W."xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/name | "Transgenic Res"xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/pages | "489-501"xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/title | "The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo."xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/24622775 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24622775 |
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