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http://purl.uniprot.org/citations/24623314http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/24623314http://www.w3.org/2000/01/rdf-schema#comment"

Background

MicroRNAs play important roles in the development and progression of various cancers. Recent studies have shown that miR-638 was downregulated in several tumors; however, its role in gastric cancer (GC) has not been investigated in detail.

Aims

The purpose of this study was to determine the role of miR-638 and to elucidate its regulatory mechanism in GC.

Methods

The expression levels of miR-638 and specificity protein 2 (Sp2) were detected by real-time PCR and Western blotting in GC. After pcDNA6.2-GW/EmGFP-miR-638 vector, miR-638 inhibitor and Sp2-siRNA transfection, the AGS cell proliferation was investigated by MTT assay and cell cycle, and apoptosis was detected using the Annexin V/PI. In addition, the regulation of Sp2 by miR-638 was evaluated by real-time RT-PCR, Western blot and luciferase reporter assays; cyclin D1 expression was measured by Western blotting.

Results

The expression of miR-638 is dramatically down-regulated and Sp2 expression is remarkably up-regulated in GC tissues. Luciferase assays revealed that miR-638 inhibited Sp2 expression by targeting the 3'-UTR of Sp2 mRNA. Overexpression of miR-638 and Sp2-siRNA reduced Sp2 expression at both the mRNA and protein levels in vitro, and inhibition of miR-638 increased Sp2 expression. Moreover, we found that miR-638 overexpression and Sp2-siRNA markedly suppressed cell proliferation with decreasing expression of cyclin D1 and inducing G1-phase cell-cycle arrest in vitro; inhibition of miR-638 significantly promoted cell proliferation by increasing expression of cyclin D1 and leading more cells into the S and G2/M phase.

Conclusions

Our results demonstrated that miR-638 suppressed GC cell proliferation by targeting Sp2 with influence on the expression of cyclin D1. We suggest that miR-638 might be a candidate predictor or an anticancer therapeutic target for GC patients."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.org/dc/terms/identifier"doi:10.1007/s10620-014-3087-5"xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Han J."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Huang C."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Yang J."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Shao Y."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Yao Y."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Zhao L.Y."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Wang X.F."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Song T.S."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/author"Tong D.D."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/name"Dig Dis Sci"xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/pages"1743-1753"xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/title"miR-638 suppresses cell proliferation in gastric cancer by targeting Sp2."xsd:string
http://purl.uniprot.org/citations/24623314http://purl.uniprot.org/core/volume"59"xsd:string
http://purl.uniprot.org/citations/24623314http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/24623314
http://purl.uniprot.org/citations/24623314http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/24623314
http://purl.uniprot.org/uniprot/#_Q02086-mappedCitation-24623314http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24623314
http://purl.uniprot.org/uniprot/#_Q9BRW5-mappedCitation-24623314http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/24623314
http://purl.uniprot.org/uniprot/Q02086http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/24623314
http://purl.uniprot.org/uniprot/Q9BRW5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/24623314