http://purl.uniprot.org/citations/24629698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24629698 | http://www.w3.org/2000/01/rdf-schema#comment | "PurposeThe present study examined the correlation of α-dystroglycan (α-DG) expression and like-acetylglucosaminyl transferase (LARGE) with metastasis of human tongue cancer.Materials and methodsFifty human tongue cancer tissues and 2 tongue squamous cell carcinoma cell lines (CAL27 and SCC4) were involved. Immunohistochemistry was used to detect the expression of α-DG and LARGE. Methylation-specific polymerase chain reaction was performed to assess the methylation status of the LARGE gene promoter. CAL27 and SCC4 cells were transfected with exogenous LARGE and treated with 5-aza-2'-deoxycytidine (Aza-dC), respectively. Glycol sites of α-DG were detected by western blotting. In addition, the laminin overlay assay, cell adhesion assay, and invasion assay were performed.ResultsImmunohistochemical results showed that decreased expression of VIA4-1 and IIH6 (antibodies that recognize the glycol sites of α-DG) were correlated with the lymph node metastasis of tongue cancer (n = 50; P = .016 and .025, respectively). Decreased LARGE expression and hypermethylation of the LARGE gene promoter were correlated with lymph node metastasis and α-DG glycosylation in human tongue cancer (n = 50; P = .043 and .015 respectively). In addition, LARGE overexpression and Aza-dC treatment actively led to restoration of functional α-DG expression, elevation of laminin binding, and decrease of migratory ability in cancer cells.ConclusionThe results suggested that absent α-DG expression and LARGE deregulation were closely associated with nodal metastasis of tongue cancer. Aberrant α-DG expression and glycosylation were attributed at least in part to the abnormal epigenetic modification of LARGE, especially the hypermethylation of its promoter."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.joms.2013.12.031"xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Shen H."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Zhu F."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Song K."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Xia X.Y."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Zhang H.Z."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/author | "Shang Z.J."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/name | "J Oral Maxillofac Surg"xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/pages | "1106-1118"xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/title | "Correlation of deregulated like-acetylglucosaminyl transferase and aberrant alpha-dystroglycan expression with human tongue cancer metastasis."xsd:string |
http://purl.uniprot.org/citations/24629698 | http://purl.uniprot.org/core/volume | "72"xsd:string |
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