| http://purl.uniprot.org/citations/24649829 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24649829 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24649829 | http://www.w3.org/2000/01/rdf-schema#comment | "GTPases (G proteins) hydrolyze the conversion of GTP to GDP and free phosphate, comprising an integral part of prokaryotic and eukaryotic signaling, protein biosynthesis and cell division, as well as membrane transport processes. The G protein cycle is brought to a halt after GTP hydrolysis, and requires the release of GDP before a new cycle can be initiated. For eukaryotic heterotrimeric Gαβγ proteins, the interaction with a membrane-bound G protein-coupled receptor catalyzes the release of GDP from the Gα subunit. Structural and functional studies have implicated one of the nucleotide binding sequence motifs, the G5 motif, as playing an integral part in this release mechanism. Indeed, a Gαs G5 mutant (A366S) was shown to have an accelerated GDP release rate, mimicking a G protein-coupled receptor catalyzed release state. In the present study, we investigate the role of the equivalent residue in the G5 motif (residue A143) in the prokaryotic membrane protein FeoB from Streptococcus thermophilus, which includes an N-terminal soluble G protein domain. The structure of this domain has previously been determined in the apo and GDP-bound states and in the presence of a transition state analogue, revealing conformational changes in the G5 motif. The A143 residue was mutated to a serine and analyzed with respect to changes in GTPase activity, nucleotide release rate, GDP affinity and structural alterations. We conclude that the identity of the residue at this position in the G5 loop plays a key role in the nucleotide release rate by allowing the correct positioning and hydrogen bonding of the nucleotide base."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.12779"xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.12779"xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Deshpande C.N."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Deshpande C.N."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Jormakka M."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Jormakka M."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Maher M.J."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Maher M.J."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Schenk G."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Schenk G."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Pedroso M.M."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Pedroso M.M."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Vincent K."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Vincent K."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Guilfoyle A.P."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/author | "Guilfoyle A.P."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/name | "FEBS J."xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/pages | "2254-2265"xsd:string |
| http://purl.uniprot.org/citations/24649829 | http://purl.uniprot.org/core/pages | "2254-2265"xsd:string |