| http://purl.uniprot.org/citations/24652291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24652291 | http://www.w3.org/2000/01/rdf-schema#comment | "Expression of CGS1, which codes for an enzyme of methionine biosynthesis, is feedback-regulated by mRNA degradation in response to S-adenosyl-L-methionine (AdoMet). In vitro studies revealed that AdoMet induces translation arrest at Ser-94, upon which several ribosomes stack behind the arrested one, and mRNA degradation occurs at multiple sites that presumably correspond to individual ribosomes in a stacked array. Despite the significant contribution of stacked ribosomes to inducing mRNA degradation, little is known about the ribosomes in the stacked array. Here, we assigned the peptidyl-tRNA species of the stacked second and third ribosomes to their respective codons and showed that they are arranged at nine-codon intervals behind the Ser-94 codon, indicating tight stacking. Puromycin reacts with peptidyl-tRNA in the P-site, releasing the nascent peptide as peptidyl-puromycin. This reaction is used to monitor the activity of the peptidyltransferase center (PTC) in arrested ribosomes. Puromycin reaction of peptidyl-tRNA on the AdoMet-arrested ribosome, which is stalled at the pre-translocation step, was slow. This limited reactivity can be attributed to the peptidyl-tRNA occupying the A-site at this step rather than to suppression of PTC activity. In contrast, puromycin reactions of peptidyl-tRNA with the stacked second and third ribosomes were slow but were not as slow as pre-translocation step ribosomes. We propose that the anticodon end of peptidyl-tRNA resides in the A-site of the stacked ribosomes and that the stacked ribosomes are stalled at an early step of translocation, possibly at the P/E hybrid state."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m113.526616"xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Fujiwara T."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Yamashita Y."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Naito S."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Onouchi H."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Satake A."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Sotta N."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Kadokura Y."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/author | "Takigawa I."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/pages | "12693-12704"xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/title | "Ribosomes in a stacked array: elucidation of the step in translation elongation at which they are stalled during S-adenosyl-L-methionine-induced translation arrest of CGS1 mRNA."xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/24652291 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24652291 |
| http://purl.uniprot.org/citations/24652291 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24652291 |
| http://purl.uniprot.org/uniprot/#_A0A178VB36-mappedCitation-24652291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24652291 |
| http://purl.uniprot.org/uniprot/#_P55217-mappedCitation-24652291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24652291 |
| http://purl.uniprot.org/uniprot/A0A178VB36 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24652291 |
| http://purl.uniprot.org/uniprot/P55217 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24652291 |