| http://purl.uniprot.org/citations/24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24659802 | http://www.w3.org/2000/01/rdf-schema#comment | "G-protein-coupled receptors (GPCRs) regulate the organisation of the actin cytoskeleton by activating the Rac subfamily of small GTPases. The guanine-nucleotide-exchange factor (GEF) P-Rex1 is engaged downstream of GPCRs and phosphoinositide 3-kinase (PI3K) in many cell types, and promotes tumorigenic signalling and metastasis in breast cancer and melanoma, respectively. Although P-Rex1-dependent functions have been attributed to its GEF activity towards Rac1, we show that P-Rex1 also acts as a GEF for the Rac-related GTPase RhoG, both in vitro and in GPCR-stimulated primary mouse neutrophils. Furthermore, loss of either P-Rex1 or RhoG caused equivalent reductions in GPCR-driven Rac activation and Rac-dependent NADPH oxidase activity, suggesting they both function upstream of Rac in this system. Loss of RhoG also impaired GPCR-driven recruitment of the Rac GEF DOCK2, and F-actin, to the leading edge of migrating neutrophils. Taken together, our results reveal a new signalling hierarchy in which P-Rex1, acting as a GEF for RhoG, regulates Rac-dependent functions indirectly through RhoG-dependent recruitment of DOCK2. These findings thus have broad implications for our understanding of GPCR signalling to Rho GTPases and the actin cytoskeleton."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.153049"xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Fukui Y."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Hawkins P.T."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Stephens L.R."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Anderson K.E."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Der C.J."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Rossman K.L."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Gambardella L."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Welch H.C."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Lawson C.D."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/author | "Damoulakis G."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/pages | "2589-2600"xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/title | "P-Rex1 directly activates RhoG to regulate GPCR-driven Rac signalling and actin polarity in neutrophils."xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://purl.uniprot.org/core/volume | "127"xsd:string |
| http://purl.uniprot.org/citations/24659802 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24659802 |
| http://purl.uniprot.org/citations/24659802 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24659802 |
| http://purl.uniprot.org/uniprot/#_A0A087WRV5-mappedCitation-24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24659802 |
| http://purl.uniprot.org/uniprot/#_A0A087WS21-mappedCitation-24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24659802 |
| http://purl.uniprot.org/uniprot/#_A0A087WRD8-mappedCitation-24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24659802 |
| http://purl.uniprot.org/uniprot/#_A0A087WRG8-mappedCitation-24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24659802 |
| http://purl.uniprot.org/uniprot/#_P52735-mappedCitation-24659802 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24659802 |