| http://purl.uniprot.org/citations/24681254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24681254 | http://www.w3.org/2000/01/rdf-schema#comment | "4-Vinylcyclohexene (VCH) is a dimer of 1,3-butadiene produced as a by-product of pesticides, plastic, rubber, flame retardants, and tire production. Although, several studies have reported the ovotoxicity of VCH, information on a possible involvement of oxidative stress in the toxicity of this occupational chemical is scarce. Hence, this study was carried out to investigate further possible mechanisms of toxicity of VCH with a specific emphasis on oxidative stress using a Drosophila melanogaster model. D. melanogaster (both genders) of 1 to 3 days old were exposed to different concentrations of VCH (10 µM-1 mM) in the diet for 5 days. Subsequently, the survival and negative geotaxis assays and the quantification of reactive oxygen species (ROS) generation were determined. In addition, we evaluated RT-PCR expressions of selected oxidative stress and antioxidant mRNA genes (HSP27, 70, and 83, SOD, Nrf-2, MAPK2, and catalase). Furthermore, catalase, glutathione-S-transferase (GST), delta aminolevulinic acid dehydratase (δ-ALA-D), and acetylcholinesterase (AChE) activities were determined. VCH exposure impaired negative geotaxic behavior and induced the mRNA of SOD, Nrf-2, and MAPK2 genes expressions. There were increases in catalase and ROS production, as well as inhibitions of GST, δ-ALA-D, and AChE activities (P<0.05). Our results suggest that the VCH mechanism of toxicity is associated with oxidative damage, as evidenced by the alteration in the oxidative stress-antioxidant balance, and possible neurotoxic consequences due to decreased AChE activity, and impairments in negative geotaxic behavior. Thus, we conclude that D. melanogaster is a useful model for investigating the toxicity of VCH exposure, and here, we have provided further insights on the mechanism of VCH-induced toxicity."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.freeradbiomed.2014.03.014"xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Henrique Lugokenski T."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Kalar Nascimento T."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Olalekan Abolaji A."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Olatunde Farombi E."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Pansera Waczuk E."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Paul Kamdem J."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "Teixeira Rocha J.B."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/author | "da Silva Loreto E.L."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/name | "Free Radic Biol Med"xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/pages | "99-108"xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/title | "Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster."xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://purl.uniprot.org/core/volume | "71"xsd:string |
| http://purl.uniprot.org/citations/24681254 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24681254 |
| http://purl.uniprot.org/citations/24681254 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24681254 |
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| http://purl.uniprot.org/uniprot/#_M9PC43-mappedCitation-24681254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24681254 |
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| http://purl.uniprot.org/uniprot/#_M9PBL3-mappedCitation-24681254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24681254 |
| http://purl.uniprot.org/uniprot/#_P13706-mappedCitation-24681254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24681254 |