| http://purl.uniprot.org/citations/24684931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24684931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24684931 | http://www.w3.org/2000/01/rdf-schema#comment | "Argonaute (AGO) proteins are key nuclease effectors of RNAi. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, which engages amplified small RNAs during RNAi. These studies identified a robust association between WAGO-1 and a conserved Vasa ATPase-related protein RDE-12. rde-12 mutants are deficient in RNAi, including viral suppression, and fail to produce amplified secondary siRNAs and certain endogenous siRNAs (endo-siRNAs). RDE-12 colocalizes with WAGO-1 in germline P granules and in cytoplasmic and perinuclear foci in somatic cells. These findings and our genetic studies suggest that RDE-12 is first recruited to target mRNA by upstream AGOs (RDE-1 and ERGO-1), where it promotes small RNA amplification and/or WAGO-1 loading. Downstream of these events, RDE-12 forms an RNase-resistant (target mRNA-independent) complex with WAGO-1 and may thus have additional functions in target mRNA surveillance and silencing."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2014.03.008"xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2014.03.008"xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Gu W."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Gu W."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Mello C.C."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Mello C.C."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Shirayama M."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Shirayama M."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Seth M."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Seth M."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Stanney W."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/author | "Stanney W."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/pages | "845-851"xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/pages | "845-851"xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/title | "The vasa homolog rde-12 engages target mRNA and multiple argonaute proteins to promote RNAi in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/title | "The vasa homolog rde-12 engages target mRNA and multiple argonaute proteins to promote RNAi in C. elegans."xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/24684931 | http://purl.uniprot.org/core/volume | "24"xsd:string |