| http://purl.uniprot.org/citations/24728280 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24728280 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24728280 | http://www.w3.org/2000/01/rdf-schema#comment | "Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst tethering complex, we examined whether this could be explained by a hidden gene duplication. Re-sequencing and manual assembly of the Arabidopsis thaliana SEC10 (At5g12370) locus revealed that this locus, comprising a single gene in the reference genome assembly, indeed contains two paralogous genes in tandem, SEC10a and SEC10b, and that a sequence segment of 7 kb in length is missing from the reference genome sequence. Differences between the two paralogs are concentrated in non-coding regions, while the predicted protein sequences exhibit 99% identity, differing only by substitution of five amino acid residues and an indel of four residues. Both SEC10 genes are expressed, although varying transcript levels suggest differential regulation. Homozygous T-DNA insertion mutants in either paralog exhibit a wild-type phenotype, consistent with proposed extensive functional redundancy of the two genes. By these observations we demonstrate that recently duplicated genes may remain hidden even in well-characterized genomes, such as that of A. thaliana. Moreover, we show that the use of the existing A. thaliana reference genome sequence as a guide for sequence assembly of new Arabidopsis accessions or related species has at least in some cases led to error propagation."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0094077"xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0094077"xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Elias M."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Elias M."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Cvrckova F."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Cvrckova F."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Fowler J.E."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Fowler J.E."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Cole R."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Cole R."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Synek L."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Synek L."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Vukasinovic N."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Vukasinovic N."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Zarsky V."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/author | "Zarsky V."xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/pages | "E94077"xsd:string |
| http://purl.uniprot.org/citations/24728280 | http://purl.uniprot.org/core/pages | "E94077"xsd:string |