http://purl.uniprot.org/citations/24780222 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24780222 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/24780222 | http://www.w3.org/2000/01/rdf-schema#comment | "In this work we evaluate the interaction of two optogenetic protein variants (CIB1, CIBN) with their complementary protein CRY2 by single-molecule tools in cell-free extracts. After validating the blue light induced co-localization of CRY2 and CIB1/N by Förster resonance energy transfer (FRET) in live cells, a fluorescence correlation spectroscopy (FCS) based method was developed to quantitatively determine the in vitro association of the extracted proteins. Our experiments suggest that CIB1, in comparison with CIBN, possesses a better coupling efficiency with CRY2 due to its intact protein structure and lower diffusion rate within 300s detection window."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ab.2014.04.023"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ab.2014.04.023"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Choudhury S.R."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Choudhury S.R."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Cui Y."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Cui Y."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Irudayaraj J."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/author | "Irudayaraj J."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/name | "Anal. Biochem."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/name | "Anal. Biochem."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/pages | "58-60"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/pages | "58-60"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/title | "Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/title | "Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools."xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/volume | "458"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://purl.uniprot.org/core/volume | "458"xsd:string |
http://purl.uniprot.org/citations/24780222 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24780222 |
http://purl.uniprot.org/citations/24780222 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24780222 |
http://purl.uniprot.org/citations/24780222 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24780222 |
http://purl.uniprot.org/citations/24780222 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24780222 |