| http://purl.uniprot.org/citations/24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24798333 | http://www.w3.org/2000/01/rdf-schema#comment | "Quality control (QC) in the endoplasmic reticulum (ER) scrutinizes newly synthesized proteins and directs them either to ER export or ER-associated degradation (ERAD). Here, we demonstrate that the human δ-opioid receptor (hδOR) is subjected to ERQC in both N-glycan-dependent and -independent manners. This was shown by investigating the biosynthesis and trafficking of wild-type and non-N-glycosylated F27C variants in metabolic pulse-chase assays coupled with flow cytometry and cell surface biotinylation. Both QC mechanisms distinguished the minute one-amino acid difference between the variants, targeting a large fraction of hδOR-Cys(27) to ERAD. However, the N-glycan-independent QC was unable to compensate the N-glycan-dependent pathway, and some incompletely folded non-N-glycosylated hδOR-Cys(27) reached the cell surface in conformation incompatible with ligand binding. The turnover of receptors associating with the molecular chaperone calnexin (CNX) was significantly slower for the hδOR-Cys(27), pointing to an important role of CNX in the hδOR N-glycan-dependent QC. This was further supported by the fact that inhibiting the co-translational interaction of hδOR-Cys(27) precursors with CNX led to their ERAD. Opioid receptor pharmacological chaperones released the CNX-bound receptors to ER export and, furthermore, were able to rescue the Cys(27) variant from polyubiquitination and retrotranslocation to the cytosol whether carrying N-glycans or not. Taken together, the hδOR appears to rely primarily on the CNX-mediated N-glycan-dependent QC that has the capacity to assist in folding, whereas the N-glycan-independent mechanism constitutes an alternative, although less accurate, system for directing misfolded/incompletely folded receptors to ERAD, possibly in altered cellular conditions."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m114.566273"xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/author | "Bouvier M."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/author | "Lackman J.J."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/author | "Petaja-Repo U.E."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/author | "Hogue M."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/author | "Markkanen P.M."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/pages | "17830-17842"xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/title | "N-Glycan-dependent and -independent quality control of human delta opioid receptor N-terminal variants."xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/24798333 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24798333 |
| http://purl.uniprot.org/citations/24798333 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24798333 |
| http://purl.uniprot.org/uniprot/#_A0A346G5W5-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
| http://purl.uniprot.org/uniprot/#_A0A346G5W6-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
| http://purl.uniprot.org/uniprot/#_A0A346G5W7-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
| http://purl.uniprot.org/uniprot/#_A0A346G5W8-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
| http://purl.uniprot.org/uniprot/#_D2CGC9-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
| http://purl.uniprot.org/uniprot/#_P41143-mappedCitation-24798333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24798333 |
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| http://purl.uniprot.org/uniprot/A0A346G5W8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/24798333 |
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