| http://purl.uniprot.org/citations/24801387 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24801387 | http://www.w3.org/2000/01/rdf-schema#comment | "The present project was designed to investigate phosphorylation of p70S6K1 in an animal model of skeletal muscle overload. Within 24 h of male Sprague-Dawley rats undergoing unilateral tenotomy to induce functional overloading of the plantaris muscle, phosphorylation of the Thr³⁸⁹ and Thr⁴²¹/Ser⁴²⁴ sites on p70S6K1 was significantly elevated. Since the Thr⁴²¹/Ser⁴²⁴ sites are purportedly mammalian target of rapamycin complex 1 (mTORC1) independent, we sought to identify the kinase(s) responsible for their phosphorylation. Initially, we used IGF-I treatment of serum-deprived HEK-293E cells as an in vitro model system, because IGF-I promotes phosphorylation of p70S6K1 on both the Thr³⁸⁹ and Thr⁴²¹/Ser⁴²⁴ sites in skeletal muscle and in cells in culture. We found that, whereas the mTOR inhibitor TORIN2 prevented the IGF-I-induced phosphorylation of the Thr⁴²¹/Ser⁴²⁴ sites, it surprisingly enhanced phosphorylation of these sites during serum deprivation. JNK inhibition with SP600125 attenuated phosphorylation of the Thr⁴²¹/Ser⁴²⁴ sites, and in combination with TORIN2 both the effect of IGF-I and the enhanced Thr⁴²¹/Ser⁴²⁴ phosphorylation during serum deprivation were ablated. In contrast, both JNK activation with anisomycin and knockdown of the mTORC2 subunit rictor specifically stimulated phosphorylation of the Thr⁴²¹/Ser⁴²⁴ sites, suggesting that mTORC2 represses JNK-mediated phosphorylation of these sites. The role of JNK in mediating p70S6K1 phosphorylation was confirmed in the animal model noted above, where rats treated with SP600125 exhibited attenuated Thr⁴²¹/Ser⁴²⁴ phosphorylation. Overall, the results provide evidence that the mTORC1 and JNK signaling pathways coordinate the site-specific phosphorylation of p70S6K1. They also identify a novel role for mTORC1 and mTORC2 in the inhibition of JNK."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpendo.00064.2014"xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/author | "Martin T.D."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/author | "Dennis M.D."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/author | "Jefferson L.S."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/author | "Kimball S.R."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/author | "Gordon B.S."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/name | "Am J Physiol Endocrinol Metab"xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/pages | "E1397-405"xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/title | "mTORC1 and JNK coordinate phosphorylation of the p70S6K1 autoinhibitory domain in skeletal muscle following functional overloading."xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://purl.uniprot.org/core/volume | "306"xsd:string |
| http://purl.uniprot.org/citations/24801387 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24801387 |
| http://purl.uniprot.org/citations/24801387 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24801387 |
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| http://purl.uniprot.org/uniprot/#_P49185-mappedCitation-24801387 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24801387 |
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