| http://purl.uniprot.org/citations/24811221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24811221 | http://www.w3.org/2000/01/rdf-schema#comment | "The far-upstream element-binding protein-interacting repressor (FIR) is a c-myc transcriptional suppressor. FIR is alternatively spliced to lack the transcriptional repression domain within exon 2 (FIRΔexon2) in colorectal cancers. FIR and FIRΔexon2 form homo- or heterodimers that complex with SAP155. SAP155, a subunit of the essential splicing factor 3b subcomplex in the spliceosome, is required for proper P27Kip1 pre-mRNA splicing, and P27Kip1 arrests cells at G1. In contrast, FIR was co-immunoprecipitated with Ku86 and DNA-PKcs. siRNA against Ku86/Ku70 decreased FIR and P27Kip1 expression, whereas siRNA against FIR decreased Ku86/XRCC5 and P27Kip1 expression. Thus the mechanical interaction of FIR/FIRΔexon2/SAP155 bridges c-myc and P27Kip1 expression, potentially integrates cell-cycle progression and c-myc transcription in cell. Bleomycin(BLM) is an anticancer agent that introduces DNA breaks. Because DNA breaks generate the recruitment of Ku86/Ku70 to bind to the broken DNA ends, the possible involvement of FIR and Ku86/Ku70 interaction in the BLM-induced DNA damage repair response was investigated in this study. First, BLM treatment reduced SAP155 expression and increased FIR and FIRΔexon2 mRNA expression as well as the ratio of FIRΔexon2:FIR in hepatoblastoma cells (HLE and HLF). Second, FIR or FIRΔexon2 adenovirus vectors (Ad-FIR or Ad-FIRΔexon2) increased Ku86/Ku70 and P27Kip1 expression in vitro. Third, BLM decreased P27Kip1 protein expression, whereas increased P27Kip1 and γH2AX expression with Ad-FIRΔexon2. Together, the interaction of FIR/SAP155 modulates FIR splicing and involves in cell-cycle control or cell fate via P27Kip1 and c-myc in BLM-induced DNA damage pathway. This novel function of FIR splicing will contribute to clinical studies of cancer management through elucidating the mechanical interaction of FIR/FIRΔexon2/SAP155 as a potential target for cancer treatment."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.org/dc/terms/identifier | "doi:10.18632/oncotarget.1650"xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Kubo S."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Matsushita K."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Miyazaki M."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Shimada H."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Ohtsuka M."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Nomura F."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Satoh M."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Tsuchida S."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Seimiya M."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/author | "Rahmutulla B."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/name | "Oncotarget"xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/pages | "2404-2417"xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/title | "Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway."xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/24811221 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24811221 |
| http://purl.uniprot.org/citations/24811221 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24811221 |
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| http://purl.uniprot.org/uniprot/#_A0A994J6K6-mappedCitation-24811221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24811221 |
| http://purl.uniprot.org/uniprot/#_B4DGZ4-mappedCitation-24811221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24811221 |
| http://purl.uniprot.org/uniprot/#_A0PJH5-mappedCitation-24811221 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24811221 |