| http://purl.uniprot.org/citations/24814819 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24814819 | http://www.w3.org/2000/01/rdf-schema#comment | "Recent evidence suggests that the predominant astrocyte glutamate transporter, GLT-1/ Excitatory Amino Acid Transporter 2 (EAAT2) is associated with mitochondria. We used primary cultures of mouse astrocytes to assess co-localization of GLT-1 with mitochondria, and tested whether the interaction was dependent on neurons, actin polymerization or the kinesin adaptor, TRAK2. Mouse primary astrocytes were transfected with constructs expressing V5-tagged GLT-1, pDsRed1-Mito with and without dominant negative TRAK2. Astrocytes were visualized using confocal microscopy and co-localization was quantified using Volocity software. Image analysis of confocal z-stacks revealed no co-localization between mitochondria and GLT-1 in pure astrocyte cultures. Co-culture of astrocytes with primary mouse cortical neurons revealed more mitochondria in processes and a positive correlation between mitochondria and GLT-1. This co-localization was not further enhanced after neuronal depolarization induced by 1 h treatment with 15 mM K(+). In pure astrocytes, a rho kinase inhibitor, Y27632 caused the distribution of mitochondria to astrocyte processes without enhancing GLT-1/mitochondrial co-localization, however, in co-cultures, Y27632 abolished mitochondrial:GLT-1 co-localization. Disrupting potential mitochondrial: kinesin interactions using dominant negative TRAK2 did not alter GLT-1 distribution or GLT-1: mitochondrial co-localization. We conclude that the association between GLT-1 and mitochondria is modest, is driven by synaptic activity and dependent on polymerized actin filaments. Mitochondria have limited co-localization with the glutamate transporter GLT-1 in primary astrocytes in culture. Few mitochondria are in the fine processes where GLT-1 is abundant. It is necessary to culture astrocytes with neurones to drive a significant level of co-localization, but co-localization is not further altered by depolarization, manipulating sodium ion gradients or Na/K ATPase activity."xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.org/dc/terms/identifier | "doi:10.1111/jnc.12759"xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/author | "Rattray M."xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/author | "Hirst W.D."xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/author | "Ugbode C.I."xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/name | "J Neurochem"xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/pages | "668-677"xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/title | "Neuronal influences are necessary to produce mitochondrial co-localization with glutamate transporters in astrocytes."xsd:string |
| http://purl.uniprot.org/citations/24814819 | http://purl.uniprot.org/core/volume | "130"xsd:string |
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