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| http://purl.uniprot.org/citations/24846647 | http://www.w3.org/2000/01/rdf-schema#comment | "Tubulin protomers undergo an extensive array of post-translational modifications to tailor microtubules to specific tasks. One such modification, the acetylation of lysine 40 of α-tubulin, located in the lumen of microtubules, is associated with stable, long-living microtubule structures. MEC-17 was recently identified as the acetyltransferase that mediates this event. We have determined the crystal structure of the catalytic core of human MEC-17 in complex with its cofactor acetyl-CoA at 1.7Å resolution. The structure reveals that the MEC-17 core adopts a canonical Gcn5-related N-acetyltransferase (GNAT) fold that is decorated with extensive surface loops. An enzymatic analysis of 33 MEC-17 surface mutants identifies hot-spot residues for catalysis and substrate recognition. A large, evolutionarily conserved hydrophobic surface patch that is critical for enzymatic activity is identified, suggesting that specificity is achieved by interactions with the α-tubulin substrate that extend outside of the modified surface loop. An analysis of MEC-17 mutants in Caenorhabditis elegans shows that enzymatic activity is dispensable for touch sensitivity."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2014.05.009"xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Hoelz A."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Sternberg P.W."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Davenport A.M."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Collins L.N."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Chiu H."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/author | "Minor P.J."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/pages | "2605-2616"xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/title | "Structural and functional characterization of the alpha-tubulin acetyltransferase MEC-17."xsd:string |
| http://purl.uniprot.org/citations/24846647 | http://purl.uniprot.org/core/volume | "426"xsd:string |
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