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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/24901643 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24901643 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24901643 | http://www.w3.org/2000/01/rdf-schema#comment | "The "silent" voltage-gated potassium (KvS) channel subunit Kv6.4 does not form electrically functional homotetramers at the plasma membrane but assembles with Kv2.1 subunits, generating functional Kv2.1/Kv6.4 heterotetramers. The N-terminal T1 domain determines the subfamily-specific assembly of Kv1-4 subunits by preventing interactions between subunits that belong to different subfamilies. For Kv6.4, yeast-two-hybrid experiments showed an interaction of the Kv6.4 N-terminus with the Kv2.1 N-terminus, but unexpectedly also with the Kv3.1 N-terminus. We confirmed this interaction by Fluorescence Resonance Energy Transfer (FRET) and co-immunoprecipitation (co-IP) using N-terminal Kv3.1 and Kv6.4 fragments. However, full-length Kv3.1 and Kv6.4 subunits do not form heterotetramers at the plasma membrane. Therefore, additional interactions between the Kv6.4 and Kv2.1 subunits should be important in the Kv2.1/Kv6.4 subfamily-specificity. Using FRET and co-IP approaches with N- and C-terminal fragments we observed that the Kv6.4 C-terminus physically interacts with the Kv2.1 N-terminus but not with the Kv3.1 N-terminus. The N-terminal amino acid sequence CDD which is conserved between Kv2 and KvS subunits appeared to be a key determinant since charge reversals with arginine substitutions abolished the interaction between the N-terminus of Kv2.1 and the C-terminus of both Kv2.1 and Kv6.4. In addition, the Kv6.4(CKv3.1) chimera in which the C-terminus of Kv6.4 was replaced by the corresponding domain of Kv3.1, disrupted the assembly with Kv2.1. These results indicate that the subfamily-specific Kv2.1/Kv6.4 heterotetramerization is determined by interactions between Kv2.1 and Kv6.4 that involve both the N- and C-termini in which the conserved N-terminal CDD sequence plays a key role."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0098960"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0098960"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Timmermans J.P."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Timmermans J.P."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Snyders D.J."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Snyders D.J."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Mayeur E."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Mayeur E."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Bocksteins E."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Bocksteins E."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Regnier G."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Regnier G."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Van Tilborg A."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/author | "Van Tilborg A."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/pages | "E98960"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/pages | "E98960"xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/title | "The subfamily-specific interaction between Kv2.1 and Kv6.4 subunits is determined by interactions between the N- and C-termini."xsd:string |
| http://purl.uniprot.org/citations/24901643 | http://purl.uniprot.org/core/title | "The subfamily-specific interaction between Kv2.1 and Kv6.4 subunits is determined by interactions between the N- and C-termini."xsd:string |