| http://purl.uniprot.org/citations/24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24945770 | http://www.w3.org/2000/01/rdf-schema#comment | "Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function; however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical neuronal migration phenotype. Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx knockouts akin to the effect in wild-type mice, suggesting shRNA-mediated off-target toxicity. This effect was not limited to Dcx, as it was observed in Dclk1 knockouts, as well as with a fraction of scrambled shRNAs, suggesting a sequence-dependent but not sequence-specific effect. Profiling RNAs from electroporated cells showed a defect in endogenous let7 miRNA levels, and disruption of let7 or Dicer recapitulated the migration defect. The results suggest that shRNA-mediated knockdown can produce untoward migration effects by altering endogenous miRNA pathways."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neuron.2014.04.036"xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Fenstermaker A.G."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Gleeson J.G."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Novarino G."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Lee J.E."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Bielas S.L."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Baek S.T."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/author | "Kerjan G."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/name | "Neuron"xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/pages | "1255-1262"xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/title | "Off-target effect of doublecortin family shRNA on neuronal migration associated with endogenous microRNA dysregulation."xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://purl.uniprot.org/core/volume | "82"xsd:string |
| http://purl.uniprot.org/citations/24945770 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24945770 |
| http://purl.uniprot.org/citations/24945770 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JGH1-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JGQ6-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JGR9-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JE54-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JEN4-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A1Y7VJD6-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A0A8F5XVB9-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |
| http://purl.uniprot.org/uniprot/#_A3KN70-mappedCitation-24945770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24945770 |