| http://purl.uniprot.org/citations/24954722 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24954722 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24954722 | http://www.w3.org/2000/01/rdf-schema#comment | "The exploitation of catalytic promiscuity and the application of de novo design have recently opened the access to novel, non-natural enzymatic activities. Here we describe a structural bioinformatic method for predicting catalytic activities of enzymes based on three-dimensional constellations of functional groups in active sites ('catalophores'). As a proof-of-concept we identify two enzymes with predicted promiscuous ene-reductase activity (reduction of activated C-C double bonds) and compare them with known ene-reductases, that is, members of the Old Yellow Enzyme family. Despite completely different amino acid sequences, overall structures and protein folds, high-resolution crystal structures reveal equivalent binding modes of typical Old Yellow Enzyme substrates and ligands. Biochemical and biocatalytic data show that the two enzymes indeed possess ene-reductase activity and reveal an inverted stereopreference compared with Old Yellow Enzymes for some substrates. This method could thus be a tool for the identification of viable starting points for the development and engineering of novel biocatalysts."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.org/dc/terms/identifier | "doi:10.1038/NCOMMS5150"xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms5150"xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Gruber K."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Gruber K."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Lyskowski A."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Lyskowski A."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Pavkov-Keller T."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Pavkov-Keller T."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Schwab H."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Schwab H."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Steiner K."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Steiner K."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Macheroux P."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Macheroux P."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Steinkellner G."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Steinkellner G."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Winkler C."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Winkler C."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Oberer M."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Oberer M."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Faber K."xsd:string |
| http://purl.uniprot.org/citations/24954722 | http://purl.uniprot.org/core/author | "Faber K."xsd:string |