| http://purl.uniprot.org/citations/2496109 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2496109 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2496109 | http://www.w3.org/2000/01/rdf-schema#comment | "We describe the genetic and transcriptional organization of the promoter-distal portion of the Bacillus subtilis alpha operon. By DNA sequence analysis of the region surrounding rpoA, the gene for the alpha core subunit of RNA polymerase, we identified six open reading frames by the similarity of their products to their counterparts in the Escherichia coli transcriptional and translational apparatus. Gene order in this region, given by gene product, was IF1-B-S13-S11-alpha-L17. Gene order in E. coli is similar but not identical: SecY-B-S13-S11-S4-alpha-L17. The B. subtilis alpha region differed most strikingly from E. coli in the presence of IF1 and the absence of ribosomal protein S4, which is the translational regulator of the E. coli alpha operon. In place of the gene for S4, B. subtilis had a 177-base-pair intercistronic region containing two possible promoter sequences. However, experiments with S1 mapping of in vivo transcripts, gene disruptions in the alpha region, and a single-copy transcriptional fusion vector all suggested that these possible promoters were largely inactive during logarithmic growth, that the major promoter for the alpha operon lay upstream from the region cloned, and that the genes in the IF1 to L17 interval were cotranscribed. Thus, the transcriptional organization of the region resembles that of E. coli, wherein the alpha operon is transcribed primarily from the upstream spc promoter, but the absence of the S4 gene suggests that the translational regulation of the region may differ more fundamentally."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.171.5.2553-2562.1989"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.171.5.2553-2562.1989"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Price C.W."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Price C.W."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Thomas S.M."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Thomas S.M."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Boylan S.A."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Boylan S.A."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Suh J.-W."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/author | "Suh J.-W."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/pages | "2553-2562"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/pages | "2553-2562"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/title | "Gene encoding the alpha core subunit of Bacillus subtilis RNA polymerase is cotranscribed with the genes for initiation factor 1 and ribosomal proteins B, S13, S11, and L17."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/title | "Gene encoding the alpha core subunit of Bacillus subtilis RNA polymerase is cotranscribed with the genes for initiation factor 1 and ribosomal proteins B, S13, S11, and L17."xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/volume | "171"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://purl.uniprot.org/core/volume | "171"xsd:string |
| http://purl.uniprot.org/citations/2496109 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2496109 |
| http://purl.uniprot.org/citations/2496109 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2496109 |