| http://purl.uniprot.org/citations/24974728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24974728 | http://www.w3.org/2000/01/rdf-schema#comment | "Stem-cell antigen 1-positive (Sca-1+) cardiac stem cells (CSCs), a vital kind of CSCs in humans, promote cardiac repair in vivo and can differentiate to cardiomyocytes with 5'-azacytizine treatment in vitro. However, the underlying molecular mechanisms are unknown. β-arrestin2 is an important scaffold protein and highly expressed in the heart. To explore the function of β-arrestin2 in Sca-1+ CSC differentiation, we used β-arrestin2-knockout mice and overexpression strategies. Real-time PCR revealed that β-arrestin2 promoted 5'-azacytizine-induced Sca-1+ CSC differentiation in vitro. Because the microRNA 155 (miR-155) may regulate β-arrestin2 expression, we detected its role and relationship with β-arrestin2 and glycogen synthase kinase 3 (GSK3β), another probable target of miR-155. Real-time PCR revealed that miR-155, inhibited by β-arrestin2, impaired 5'-azacytizine-induced Sca-1+ CSC differentiation. On luciferase report assay, miR-155 could inhibit the activity of β-arrestin2 and GSK3β, which suggests a loop pathway between miR-155 and β-arrestin2. Furthermore, β-arrestin2-knockout inhibited the activity of GSK3β. Akt, the upstream inhibitor of GSK3β, was inhibited in β-arrestin2-Knockout mice, so the activity of GSK3β was regulated by β-arrestin2 not Akt. We transplanted Sca-1+ CSCs from β-arrestin2-knockout mice to mice with myocardial infarction and found similar protective functions as in wild-type mice but impaired arterial elastance. Furthermore, low level of β-arrestin2 agreed with decreased phosphorylation of AKT and increased phophorylation of GSK3β, similar to in vitro findings. The β-arrestin2/miR-155/GSK3β pathway may be a new mechanism with implications for treatment of heart disease."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.org/dc/terms/identifier | "doi:10.1111/jcmm.12339"xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Feng Y."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Yan H."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Yin D."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Stuart C."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/author | "Chua B."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/name | "J Cell Mol Med"xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/pages | "1562-1570"xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/title | "beta-arrestin2/miR-155/GSK3beta regulates transition of 5'-azacytizine-induced Sca-1-positive cells to cardiomyocytes."xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/24974728 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/24974728 |
| http://purl.uniprot.org/citations/24974728 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/24974728 |
| http://purl.uniprot.org/uniprot/Q91YI4#attribution-15A1C78797703CFD48FCD892EF8A078F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24974728 |
| http://purl.uniprot.org/uniprot/Q91YI4#attribution-1EC92A7FFCF36239D7B0936D442B1598 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24974728 |
| http://purl.uniprot.org/uniprot/Q91YI4#attribution-952C3153D1801774D0A55E04792EEACA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24974728 |
| http://purl.uniprot.org/uniprot/Q9WV60#attribution-15A1C78797703CFD48FCD892EF8A078F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/24974728 |
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| http://purl.uniprot.org/uniprot/#_J3QN53-mappedCitation-24974728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/24974728 |