| http://purl.uniprot.org/citations/24981893 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/24981893 | http://www.w3.org/2000/01/rdf-schema#comment | "Neisseria meningitidis is a leading cause of bacterial meningitis and sepsis, and its capsular polysaccharides (CPS) are a major virulence factor in meningococcal infections and form the basis for serogroup designation and protective vaccines. We formulated a novel nanovaccine containing meningococcal CPS as an antigen encapsulated in albumin-based nanoparticles (NPs) that does not require chemical conjugation to a protein carrier. These nanoparticles are taken up by antigen-presenting cells and act as antigen depot by slowly releasing the antigen. In this study, we determined the ability of CPS-loaded vaccine nanoparticles to induce co-stimulatory molecules, namely CD80, CD86, and CD95 that impact effective antigen presentation. Co-stimulatory molecule gene induction and surface expression on macrophages and dendritic cells pulsed with meningococcal CPS-loaded nanoparticles were investigated using gene array and flow cytometry methods. Meningococcal CPS-loaded NP significantly induced the surface protein expression of CD80 and CD86, markers of dendritic cell maturation, in human THP-1 macrophages and in murine dendritic cells DC2.4 in a dose-dependent manner. The massive upregulation was also observed at the gene expression. However, high dose of CPS-loaded NP, but not empty NP, induced the expression of death receptor CD95 (Fas) leading to reduced TNF-α release and reduction in cell viability. The data suggest that high expression of CD95 may lead to death of antigen-presenting cells and consequently suboptimal immune responses to vaccine. The CPS-loaded NP induces the expression of co-stimulatory molecules and acts as antigen depot and can spare antigen dose, highly desirable criteria for vaccine formulations."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.org/dc/terms/identifier | "doi:10.1208/s12248-014-9635-2"xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/author | "Zughaier S.M."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/author | "D'Souza M.J."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/author | "Gala R.P."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/author | "Ubale R.V."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/name | "AAPS J"xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/pages | "986-993"xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/title | "Induction of death receptor CD95 and co-stimulatory molecules CD80 and CD86 by meningococcal capsular polysaccharide-loaded vaccine nanoparticles."xsd:string |
| http://purl.uniprot.org/citations/24981893 | http://purl.uniprot.org/core/volume | "16"xsd:string |
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