| http://purl.uniprot.org/citations/25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25017576 | http://www.w3.org/2000/01/rdf-schema#comment | "Cerebral ischemia/reperfusion (I/R) can induce neuronal death, particularly in the hippocampal formation (HF). Molecular genetic studies have suggested that the activities of the transcription factor, hypoxia-inducible factor-1α (HIF-1α), are closely linked to ischemia-induced neuronal death. However, the mechanisms through which HIF-1α functions remain poorly understood. In this study, primary cortical neurons were subjected to oxygen‑glucose deprivation (OGD) to establish a cell model of OGD/reperfusion (RP). HIF-1α mRNA and protein expression was measured by qRT-PCR and western blot analysis. Cell proliferation was detected by MTT assay. Flow cytometric analysis was used to detect cell apoptosis and changes in mitochondrial mass. The expression of LC3-Ⅰ and LC3-Ⅱ was examined by western blot analysis. We found that HIF-1α increased cell proliferation and decreased cell apoptosis in our cell model of OGD/RP using cultured neonatal rat cortical neurons. The overexpression of HIF-1α significantly induced changes in mitochondrial mass and mitochondrial autophagy in cortical neurons. Moreover, the inhibition of HIF-1α markedly suppressed cell proliferation and mitochondrial autophagy. We also demonstrated that the HIF-1α-induced mitochondrial autophagy was accompanied by the inhibition of the mTOR pathway. This study provides direct in vitro evidence that HIF-1α overexpression triggers mitochondrial autophagy, thereby increasing neuronal survival. Our results highlight a novel target molecule toward which anti-ischemic neuroprotective effects can be applied."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.org/dc/terms/identifier | "doi:10.3892/ijmm.2014.1850"xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Dai X."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Hou L."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Hu L."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Yin L."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Bai S."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/author | "Gong G."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/name | "Int J Mol Med"xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/pages | "1133-1140"xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/title | "Upregulation of HIF-1alpha protein induces mitochondrial autophagy in primary cortical cell cultures through the inhibition of the mTOR pathway."xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://purl.uniprot.org/core/volume | "34"xsd:string |
| http://purl.uniprot.org/citations/25017576 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25017576 |
| http://purl.uniprot.org/citations/25017576 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25017576 |
| http://purl.uniprot.org/uniprot/#_A6HC66-mappedCitation-25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25017576 |
| http://purl.uniprot.org/uniprot/#_A6HC67-mappedCitation-25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25017576 |
| http://purl.uniprot.org/uniprot/#_A6IU70-mappedCitation-25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25017576 |
| http://purl.uniprot.org/uniprot/#_P42346-mappedCitation-25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25017576 |
| http://purl.uniprot.org/uniprot/#_O35800-mappedCitation-25017576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25017576 |
| http://purl.uniprot.org/uniprot/A6HC66 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25017576 |