| http://purl.uniprot.org/citations/25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25046655 | http://www.w3.org/2000/01/rdf-schema#comment | "Skin tension may influence keloid scar behavior, development, and spreading, e.g., butterfly-shaped keloid disease in the sternum. Here, we developed a three-dimensional (3D) in vitro model to mimic in vivo tension and evaluate keloid fibroblast (KF) behavior and extracellular matrix synthesis under tension. In vivo skin tension measured in volunteers (n = 4) using 3D image photogrammetry enabled prediction of actual force (35 mN). A novel cell force monitor applied tension in a fibroblast-populated 3D collagen lattice replicating the in vivo force. The effect of tension on keloid (n = 10) fibroblast (KF) and normal skin (n = 10) fibroblasts (NF) at set time points (6, 12, and 24 hours) was measured in Hsp27, PAI-2, and α2β1 integrin, tension-related genes demonstrating significant (p < 0.05) time-dependent regulation of these genes in NF vs. KF with and without tension. KF showed higher (p < 0.05) proliferation post-tension. Knockdown of all three genes in 24 and 48 hours with and without tension showed significant down-regulation in NF vs. KF. Additionally, we show significant (p < 0.05) modification of the expression of extracellular matrix-related genes post-tension following down-regulation of Hsp27, PAI-2, or α2β1 integrin. Finally, we demonstrate significant alteration in NF compared with KF morphology following knockdown. In conclusion, this study shows induction of tension-related genes expression following mechano-regulation in KFs, with potential relevance to its development and therapy."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.org/dc/terms/identifier | "doi:10.1111/wrr.12215"xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Mandal P."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Bayat A."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Syed F."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Suarez E."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Walmsley A."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/author | "Rasgado T.A."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/name | "Wound Repair Regen"xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/pages | "557-568"xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/title | "Skin equivalent tensional force alters keloid fibroblast behavior and phenotype."xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/25046655 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25046655 |
| http://purl.uniprot.org/citations/25046655 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25046655 |
| http://purl.uniprot.org/uniprot/#_A0A0X8AZ85-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_A0A8E9ZT92-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_A0A8E9ZUD7-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_B2R7Y0-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_B4DL87-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_C9J3N8-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_F1C627-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_F1C628-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |
| http://purl.uniprot.org/uniprot/#_F1C629-mappedCitation-25046655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25046655 |