http://purl.uniprot.org/citations/25057902 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/25057902 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectiveTo evaluate the impact of mesenchymal stem cells (MSCs) against hepatic I/R injury and explore the role of N-acetyltransferase 8 (NAT8) in the process.MethodsWe investigated the potential of injected MSCs systemically via the tail vein in healing injuried liver of the SD rat model of 70% hepatic I/R injury by measuring the biochemical and pathologic alterations. Subsequently, we evaluated the expression levels of NAT8 by western blotting in vivo. Concurrently, hydrogen peroxide (H2O2)-induced apoptosis in the human normal liver cell line L02 was performed in vitro to evaluate the protective effects of MSC conditioned medium (MSC-CM) on L02 cells. In addition, we downregulated and upregulated NAT8 expression in L02 cells and induced apoptosis by using H2O2 to study the protective role of NAT8.ResultsMSCs implantation led to a significant reduced liver enzyme levels, an advanced protection in the histopathological findings of the acutely injured liver and a significantly lower percentage of TUNEL-positive cells, which were increased after I/R injury. In vitro assays, MSC-CM inhibited hepatocyte apoptosis induced by H2O2. Moreover, overexpression or downregulation of NAT8 prevented or aggravated hepatocyte apoptosis induced by H2O2, respectively.ConclusionsMSC transplantation provides support to the I/R-injured liver by inhibiting hepatocellular apoptosis and stimulating NAT8 regeneration."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0103355"xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Li D."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Shi Q."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Fu J."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Zhuang Y."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/author | "Ju X."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/pages | "e103355"xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/title | "The role of N-acetyltransferase 8 in mesenchymal stem cell-based therapy for liver ischemia/reperfusion injury in rats."xsd:string |
http://purl.uniprot.org/citations/25057902 | http://purl.uniprot.org/core/volume | "9"xsd:string |
http://purl.uniprot.org/citations/25057902 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25057902 |
http://purl.uniprot.org/citations/25057902 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25057902 |
http://purl.uniprot.org/uniprot/#_Q9JIY7-mappedCitation-25057902 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25057902 |
http://purl.uniprot.org/uniprot/Q9JIY7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25057902 |