http://purl.uniprot.org/citations/25085593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/25085593 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundZuo-Jin-Wan (ZJW), a traditional Chinese medicine formula, has been identified to be effective against drug resistance in cancer. In the present study, we investigated the effect of ZJW on acquired oxaliplatin-resistant and the PI3K/Akt/NF-κB pathway in vitro.MethodsWe tested the dose-response relationship of ZJW on reversing drug-resistance by CCK-8 assay and flow cytometry analysis in vitro. The protein expression of P-gp, MRP-2, LRP, and ABCB1 mRNA expression level were evaluated by Western blot and quantitative RT-PCR. The activities of PI3K/Akt/NF-κB pathway were also examined with or without ZJW, including Akt, IκB, p65 and their phosphorylation expression.ResultsWe found that ZJW significantly enhanced the sensitivity of chemotherapeutic drugs and increased oxaliplatin (L-OHP)-induced cell apoptosis in a time- and dose-dependent manner. Moreover, both ZJW and a PI3K specific inhibitor (LY294002) suppressed phosphorylation of Akt (Ser473) and NF-κB, which is necessary in the activation of the PI3K/Akt/NF-κB pathway. The effect of ZJW in reversing drug-resistance and suppressing phosphorylation of Akt (Ser473) and NF-κB were weakened after treatment with a PI3K/Akt activator in HCT116/L-OHP cells.ConclusionsOur study has provided the first direct evidence that ZJW reverses drug-resistance in human colorectal cancer by blocking the PI3K/Akt/NF-κB signaling pathway, and could be considered as a useful drug for cancer therapy."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.org/dc/terms/identifier | "doi:10.1186/1472-6882-14-279"xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Feng Y."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Wang W.H."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Sui H."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Shi X.L."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Fu X.L."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Ren J.L."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Pan S.F."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Zhou L.H."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Jin B.H."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Han Z.F."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Hou F.G."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/author | "Zhu H.R."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/name | "BMC Complement Altern Med"xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/pages | "279"xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/title | "Zuo Jin Wan reverses P-gp-mediated drug-resistance by inhibiting activation of the PI3K/Akt/NF-kappaB pathway."xsd:string |
http://purl.uniprot.org/citations/25085593 | http://purl.uniprot.org/core/volume | "14"xsd:string |
http://purl.uniprot.org/citations/25085593 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25085593 |
http://purl.uniprot.org/citations/25085593 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25085593 |
http://purl.uniprot.org/uniprot/#_P31749-mappedCitation-25085593 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25085593 |