| http://purl.uniprot.org/citations/25092794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25092794 | http://www.w3.org/2000/01/rdf-schema#comment | "The yeast succinic semi-aldehyde dehydrogenase gene (SSADH; EC 1.2.1.16) was cloned and overexpressed in Escherichia coli. Based on SDS-PAGE, the molecular mass of the subunit was around 54 kDa, and the purified recombinant enzyme had a tetrameric molecular mass of ca. 200 kDa. The specific activity of the recombinant enzyme was 1.90 µM NADH formed/min/mg, and showed maximal activity at pH 8.4. The recombinant protein was highly specific for succinate semi-aldehyde (Km = 15.48 ± 0.14 µM) and could use both NAD(+) and NADP(+) as co-factors, with Km values of 579.06 ± 30.1 µM and 1.017 ± 0.46 mM, respectively. Initial velocity studies showed that NADH was a competitive inhibitor with respect to NAD(+) (Ki = 129.5 µM) but a non-competitive inhibitor with respect to succinate semi-aldehyde. Adenine nucleotides of AMP, ADP and ATP inhibited yeast SSADH activity with Ki = 1.13-10.2 mM, and showed competitive inhibition with respect to NAD(+) and mixed-competitive, non-competitive and non-competitive inhibition, respectively, with respect to succinate semi-aldehyde. The kinetic data suggest a 'ping-pong' mechanism."xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.org/dc/terms/identifier | "doi:10.1002/yea.3035"xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/author | "Cao J."xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/author | "Singh N.K."xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/author | "Locy R.D."xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/name | "Yeast"xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/pages | "411-420"xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/title | "Characterization of the recombinant succinic semi-aldehyde dehydrogenase from Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/25092794 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25092794 |
| http://purl.uniprot.org/citations/25092794 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25092794 |
| http://purl.uniprot.org/uniprot/#_P38067-mappedCitation-25092794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25092794 |
| http://purl.uniprot.org/uniprot/P38067 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/25092794 |