| http://purl.uniprot.org/citations/25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25111069 | http://www.w3.org/2000/01/rdf-schema#comment | "Sirtuins are NAD(+)-dependent deacetylases acting as sensors in metabolic pathways and stress response. In mammals there are seven isoforms. The mitochondrial sirtuin 5 is a weak deacetylase but a very efficient demalonylase and desuccinylase; however, its substrate acyl specificity has not been systematically analyzed. Herein, we investigated a carbamoyl phosphate synthetase 1 derived peptide substrate and modified the lysine side chain systematically to determine the acyl specificity of Sirt5. From that point we designed six potent peptide-based inhibitors that interact with the NAD(+) binding pocket. To characterize the interaction details causing the different substrate and inhibition properties we report several X-ray crystal structures of Sirt5 complexed with these peptides. Our results reveal the Sirt5 acyl selectivity and its molecular basis and enable the design of inhibitors for Sirt5."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.org/dc/terms/identifier | "doi:10.1002/anie.201402679"xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Scharfe M."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Steegborn C."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Roessler C."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Nowak T."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Schutkowski M."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Sippl W."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Nguyen G.T."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Gertz M."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Pannek M."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/author | "Born I."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/name | "Angew Chem Int Ed Engl"xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/pages | "10728-10732"xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/title | "Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors."xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://purl.uniprot.org/core/volume | "53"xsd:string |
| http://purl.uniprot.org/citations/25111069 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25111069 |
| http://purl.uniprot.org/citations/25111069 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25111069 |
| http://purl.uniprot.org/uniprot/#_A0A7P0TBF5-mappedCitation-25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25111069 |
| http://purl.uniprot.org/uniprot/#_P31327-mappedCitation-25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25111069 |
| http://purl.uniprot.org/uniprot/#_Q9NXA8-mappedCitation-25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25111069 |
| http://purl.uniprot.org/uniprot/#_Q6DHI5-mappedCitation-25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25111069 |
| http://purl.uniprot.org/uniprot/#_Q7Z3A0-mappedCitation-25111069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25111069 |