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http://purl.uniprot.org/citations/25112699http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25112699http://www.w3.org/2000/01/rdf-schema#comment"

Background

Differentiation between patients with peanut allergy (PA) and those with peanut sensitization (PS) who tolerate peanut but have peanut-specific IgE, positive skin prick test responses, or both represents a significant diagnostic difficulty. Previously, gene expression microarrays were successfully used to identify biomarkers and explore immune responses during PA immunotherapy.

Objective

We aimed to characterize peanut-specific responses from patients with PA, subjects with PS, and atopic children without peanut allergy (NA children).

Methods

A preliminary exploratory microarray investigation of gene expression in peanut-activated memory TH subsets from 3 children with PA and 3 NA children identified potential PA diagnostic biomarkers. Microarray findings were confirmed by using real-time quantitative PCR in 30 subjects (12 children with PA, 12 children with PS, and 6 NA children). Flow cytometry was used to identify the TH subsets involved.

Results

Among 12,257 differentially expressed genes, IL9 showed the greatest difference between children with PA and NA children (45.59-fold change, P < .001), followed by IL5 and then IL13. Notably, IL9 allowed the most accurate classification of children with PA and NA children by using a machine-learning approach with recursive feature elimination and the random forest algorithm. Skin- and gut-homing TH cells from donors with PA expressed similar TH2- and TH9-associated genes. Real-time quantitative PCR confirmed that IL9 was the highest differentially expressed gene between children with PA and NA children (23.3-fold change, P < .01) and children with PS (18.5-fold change, P < .05). Intracellular cytokine staining showed that IL-9 and the TH2-specific cytokine IL-5 are produced by distinct TH populations.

Conclusion

In this study IL9 best differentiated between children with PA and children with PS (and atopic NA children). Mutually exclusive production of IL-9 and the TH2-specific cytokine IL-5 suggests that the IL-9-producing cells belong to the recently described TH9 subset."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.org/dc/terms/identifier"doi:10.1016/j.jaci.2014.06.032"xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Cousins D.J."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Wong Y.F."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Munteanu A."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Arno M."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Stephens A.C."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Lack G."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Brough H.A."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Ciortuz L."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Makinson K."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Sudra A."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/author"Turcanu V."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/name"J Allergy Clin Immunol"xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/pages"1329-1338.e10"xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/title"IL-9 is a key component of memory TH cell peanut-specific responses from children with peanut allergy."xsd:string
http://purl.uniprot.org/citations/25112699http://purl.uniprot.org/core/volume"134"xsd:string
http://purl.uniprot.org/citations/25112699http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25112699
http://purl.uniprot.org/citations/25112699http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25112699
http://purl.uniprot.org/uniprot/#_P15248-mappedCitation-25112699http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25112699
http://purl.uniprot.org/uniprot/#_Q6NZ74-mappedCitation-25112699http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25112699
http://purl.uniprot.org/uniprot/#_Q6NZ75-mappedCitation-25112699http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/25112699
http://purl.uniprot.org/uniprot/P15248http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/25112699
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