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| http://purl.uniprot.org/citations/25117470 | http://www.w3.org/2000/01/rdf-schema#comment | "The Saccharomyces cerevisiae kinase Sat4p has been originally identified as a protein involved in salt tolerance and stabilization of plasma membrane transporters, implicating a cytoplasmic localization. Our study revealed an additional mitochondrial (mt) localization, suggesting a dual function for Sat4p. While no mt related phenotype was observed in the absence of Sat4p, its overexpression resulted in significant changes of a specific mitochondrial subproteome. As shown by a comparative two dimensional difference gel electrophoresis (2D-DIGE) approach combined with mass spectrometry, particularly two groups of proteins were affected: the iron-sulfur containing aconitase-type proteins (Aco1p, Lys4p) and the lipoamide-containing subproteome (Lat1p, Kgd2p and Gcv3p). The lipoylation sites of all three proteins could be assigned by nanoLC-MS/MS to Lys75 (Lat1p), Lys114 (Kgd2p) and Lys102 (Gcv3p), respectively. Sat4p overexpression resulted in accumulation of the delipoylated protein variants and in reduced levels of aconitase-type proteins, accompanied by a decrease in the activities of the respective enzyme complexes. We propose a regulatory role of Sat4p in the late steps of the maturation of a specific subset of mitochondrial iron-sulfur cluster proteins, including Aco1p and lipoate synthase Lip5p. Impairment of the latter enzyme may account for the observed lipoylation defects."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0103956"xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/author | "Rodel G."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/author | "Krause U."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/author | "Czupalla C."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/author | "Gey U."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/author | "Hoflack B."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/pages | "e103956"xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/title | "Proteomic analysis reveals a novel function of the kinase Sat4p in Saccharomyces cerevisiae mitochondria."xsd:string |
| http://purl.uniprot.org/citations/25117470 | http://purl.uniprot.org/core/volume | "9"xsd:string |
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