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| http://purl.uniprot.org/citations/25118247 | http://www.w3.org/2000/01/rdf-schema#comment | "Oligosaccharyltransferase is a multiprotein complex that catalyzes asparagine-linked glycosylation of diverse proteins. Using yeast genetics and glycoproteomics, we found that transient interactions between nascent polypeptide and Ost3p/Ost6p, homologous subunits of oligosaccharyltransferase, were able to modulate glycosylation efficiency in a site-specific manner in vivo. These interactions were driven by hydrophobic and electrostatic complementarity between amino acids in the peptide-binding groove of Ost3p/Ost6p and the sequestered stretch of substrate polypeptide. Based on this dependence, we used in vivo scanning mutagenesis and in vitro biochemistry to map the precise interactions that affect site-specific glycosylation efficiency. We conclude that transient binding of substrate polypeptide by Ost3p/Ost6p increases glycosylation efficiency at asparagines proximal and C-terminal to sequestered sequences. We detail a novel mode of interaction between translocating nascent polypeptide and oligosaccharyltransferase in which binding to Ost3p/Ost6p segregates a short flexible loop of glycosylation-competent polypeptide substrate that is delivered to the oligosaccharyltransferase active site for efficient modification."xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.org/dc/terms/identifier | "doi:10.1074/mcp.m114.041178"xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/author | "Schulz B.L."xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/author | "Bailey U.M."xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/author | "Jamaluddin M.F."xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/name | "Mol Cell Proteomics"xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/pages | "3286-3293"xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/title | "Oligosaccharyltransferase subunits bind polypeptide substrate to locally enhance N-glycosylation."xsd:string |
| http://purl.uniprot.org/citations/25118247 | http://purl.uniprot.org/core/volume | "13"xsd:string |
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