RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/25122472http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/25122472http://www.w3.org/2000/01/rdf-schema#comment"The proteomic composition of the Arabidopsis (Arabidopsis thaliana) Golgi apparatus is currently reasonably well documented; however, little is known about the relative abundances between different proteins within this compartment. Accurate quantitative information of Golgi resident proteins is of great importance: it facilitates a better understanding of the biochemical processes that take place within this organelle, especially those of different polysaccharide synthesis pathways. Golgi resident proteins are challenging to quantify because the abundance of this organelle is relatively low within the cell. In this study, an organelle fractionation approach targeting the Golgi apparatus was combined with a label-free quantitative mass spectrometry (data-independent acquisition method using ion mobility separation known as LC-IMS-MS(E) [or HDMS(E)]) to simultaneously localize proteins to the Golgi apparatus and assess their relative quantity. In total, 102 Golgi-localized proteins were quantified. These data show that organelle fractionation in conjunction with label-free quantitative mass spectrometry is a powerful and relatively simple tool to access protein organelle localization and their relative abundances. The findings presented open a unique view on the organization of the plant Golgi apparatus, leading toward unique hypotheses centered on the biochemical processes of this organelle."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.org/dc/terms/identifier"doi:10.1104/pp.114.245589"xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/author"Lilley K.S."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/author"Dupree P."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/author"Nikolovski N."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/author"Gatto L."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/author"Shliaha P.V."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/date"2014"xsd:gYear
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/name"Plant Physiol"xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/pages"1033-1043"xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/title"Label-free protein quantification for plant Golgi protein localization and abundance."xsd:string
http://purl.uniprot.org/citations/25122472http://purl.uniprot.org/core/volume"166"xsd:string
http://purl.uniprot.org/citations/25122472http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/25122472
http://purl.uniprot.org/citations/25122472http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/25122472
http://purl.uniprot.org/uniprot/Q9FFU6#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q8GZ17#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q9SQG2#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/O48946#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q2UVJ5#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q9C835#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q8L8W5#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/O49605#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q9FE29#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472
http://purl.uniprot.org/uniprot/Q9SZG1#attribution-14FEF2FF41B99618F86E421A5633C976http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/25122472