| http://purl.uniprot.org/citations/2512301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2512301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2512301 | http://www.w3.org/2000/01/rdf-schema#comment | "Binding of the cytoskeletal protein vinculin to talin is one of a number of interactions involved in linking F-actin to cell-matrix junctions. To identify the talin binding domain in vinculin, we expressed the NH2-terminal region of the molecule encoded by two closely similar, but distinct vinculin cDNAs, using an in vitro transcription translation system. The 5' Eco RI-Bam HI fragment of a partial 2.89-kb vinculin cDNA encodes a 45-kD polypeptide containing the first 398 amino acids of the molecule. The equivalent restriction enzyme fragment of a second vinculin cDNA (cVin5) lacks nucleotides 746-867, and encodes a 41-kD polypeptide missing amino acids 167-207. The radiolabeled 45-kD vinculin polypeptide bound to microtiter wells coated with talin, but not BSA, and binding was inhibited by unlabeled vinculin. In contrast, the 41-kD vinculin polypeptide was devoid of talin binding activity. The role of residues 167-207 in talin binding was further analyzed by making a series of deletions spanning this region, each deletion of seven amino acids contiguous with the next. Loss of residues 167-173, 174-180, 181-187, 188-194, or 195-201 resulted in a marked reduction in talin binding activity, although loss of residues 202-208 had much less effect. When the 45-kD vinculin polypeptide was expressed in Cos cells, it localized to cell matrix junctions, whereas the 41-kD polypeptide, lacking residues 167-207, was unable to do so. Interestingly, some deletion mutants with reduced ability to bind talin in vitro, were still able to localize to cell matrix junctions."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.109.6.2917"xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.109.6.2917"xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Jackson P."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Jackson P."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Patel B."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Patel B."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Critchley D.R."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Critchley D.R."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Jones P."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Jones P."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Price G.J."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Price G.J."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Lear A.L."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Lear A.L."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Ohanion V."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/author | "Ohanion V."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/pages | "2917-2927"xsd:string |
| http://purl.uniprot.org/citations/2512301 | http://purl.uniprot.org/core/pages | "2917-2927"xsd:string |