| http://purl.uniprot.org/citations/25158625 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25158625 | http://www.w3.org/2000/01/rdf-schema#comment | "Sphingosine-1-phosphate (S1P) is an intracellularly generated bioactive lipid essential for development, vascular integrity, and immunity. These functions are mediated by S1P-selective cell surface G-protein coupled receptors. S1P signaling therefore requires extracellular release of this lipid. Several cell types release S1P and evidence for both plasma membrane transporter-mediated and vesicle-dependent secretion has been presented. Platelets are an important source of S1P and can release it in response to agonists generated at sites of vascular injury. S1P release from agonist-stimulated platelets was measured in the presence of a carrier molecule (albumin) using HPLC-MS/MS. The kinetics and agonist-dependence of S1P release were similar to that of other granule cargo e.g. platelet factor IV (PF4). Agonist-stimulated S1P release was defective in platelets from Unc13d(Jinx) (Munc13-4 null) mice demonstrating a critical role for regulated membrane fusion in this process. Consistent with this observation, platelets efficiently converted fluorescent NBD-sphingosine to its phosphorylated derivative which accumulated in granules. Fractionation of platelet organelles revealed the presence of S1P in both the plasma membrane and in α-granules. Resting platelets contained a second pool of constitutively releasable S1P that was more rapidly labeled by exogenously added sphingosine. Our studies indicate that platelets contain two pools of S1P that are released extracellularly: a readily-exchangeable, metabolically active pool of S1P, perhaps in the plasma membrane, and a granular pool that requires platelet activation and regulated exocytosis for release."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2014.08.013"xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/author | "Morris A.J."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/author | "Sunkara M."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/author | "Whiteheart S.W."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/author | "Jonnalagadda D."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/pages | "1581-1589"xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/title | "Granule-mediated release of sphingosine-1-phosphate by activated platelets."xsd:string |
| http://purl.uniprot.org/citations/25158625 | http://purl.uniprot.org/core/volume | "1841"xsd:string |
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