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| http://purl.uniprot.org/citations/25190460 | http://www.w3.org/2000/01/rdf-schema#comment | "Ribosome biogenesis is a dynamic multistep process, many features of which are still incompletely documented. Here, we show that changes in this pathway can be captured and annotated by means of a graphic set of pre-rRNA ratios, a technique we call Ratio Analysis of Multiple Precursors (RAMP). We find that knocking down a ribosome synthesis factor produces a characteristic RAMP profile that exhibits consistency across a range of depletion levels. This facilitates the inference of affected steps and simplifies comparative analysis. We applied RAMP to examine how endonucleolytic cleavages of the mouse pre-rRNA transcript in the internal transcribed spacer 1 (ITS1) are affected by depletion of factors required for maturation of the small ribosomal subunit (Rcl1, Fcf1/Utp24, Utp23) and the large subunit (Pes1, Nog1). The data suggest that completion of early maturation in a subunit triggers its release from the common pre-rRNA transcript by stimulating cleavage at the proximal site in ITS1. We also find that splitting of pre-rRNA in the 3' region of ITS1 is prevalent in adult mouse tissues and quiescent cells, as it is in human cells. We propose a model for subunit separation during mammalian ribosome synthesis and discuss its implications for understanding pre-rRNA processing pathways."xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gku787"xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/author | "Wang M."xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/author | "Pestov D.G."xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/author | "Anikin L."xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/pages | "11180-11191"xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/title | "Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis."xsd:string |
| http://purl.uniprot.org/citations/25190460 | http://purl.uniprot.org/core/volume | "42"xsd:string |
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