| http://purl.uniprot.org/citations/25203508 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25203508 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25203508 | http://www.w3.org/2000/01/rdf-schema#comment | "Mitochondrial fatty acid synthesis (mtFAS) is essential for respiratory growth in yeast and mammalian embryonic survival. The human 3-ketoacyl-acyl carrier protein (ACP) reductase (KAR) of mtFAS is a heterotetrameric α2β2-assembly composed of 17β-hydroxysteroid dehydrogenase type-8 (HSD17B8, α-subunit) and carbonyl reductase type-4 (CBR4, β-subunit). Here we provide a structural explanation for the stability of the heterotetramer from the crystal structure with NAD(+) and NADP(+) bound to the HSD17B8 and CBR4 subunits, respectively, and show that the catalytic activity of the NADPH- and ACP-dependent CBR4 subunit is crucial for a functional HsKAR. Therefore, mtFAS is NADPH- and ACP dependent, employing the 3R-hydroxyacyl-ACP intermediate. HSD17B8 assists in the formation of the competent HsKAR assembly. The intrinsic NAD(+)- and CoA-dependent activity of the HSD17B8 subunit on the 3R-hydroxyacyl-CoA intermediates may indicate a role for this subunit in routing 3R-hydroxyacyl-CoA esters, potentially arising from the metabolism of unsaturated fatty acids, into the mitochondrial β-oxidation pathway."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms5805"xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms5805"xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Wierenga R.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Wierenga R.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Hiltunen J.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Hiltunen J.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Kastaniotis A.J."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Kastaniotis A.J."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Venkatesan R."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Venkatesan R."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Jiang G."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Jiang G."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Awoniyi L.O."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Awoniyi L.O."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Prus P."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Prus P."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Sah-Teli S.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/author | "Sah-Teli S.K."xsd:string |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25203508 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |