| http://purl.uniprot.org/citations/25204428 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25204428 | http://www.w3.org/2000/01/rdf-schema#comment | "Voltage-gated, CaV2.3 calcium channels and neurokinin-1 (NK1) receptors are both present in nuclei of the central nervous system. When transiently coexpressed in human embryonic kidney (HEK) 293 cells, CaV2.3 is primarily inhibited during strong, agonist-dependent activation of NK1 receptors. NK1 receptors localize to plasma membrane rafts, and their modulation by Gq/11 protein-coupled signaling is sensitive to plasma membrane cholesterol. Here, we show that inhibition of CaV2.3 by NK1 receptors is attenuated following methyl-β-cyclodextrin (MBCD)-mediated depletion of membrane cholesterol. By contrast, inhibition of CaV2.3 was unaffected by intracellular diffusion of caveolin-1 scaffolding peptide or by overexpression of caveolin-1. Interestingly, MΒCD treatment had no effect on the macroscopic biophysical properties of CaV2.3, though it significantly decreased whole-cell membrane capacitance. Our data indicate that (1) cholesterol supports at least one component of the NK1 receptor-linked signaling pathway that inhibits CaV2.3 and (2) caveolin-1 is dispensable within this pathway. Our findings suggest that NK1 receptors reside within non-caveolar membrane rafts and that CaV2.3 resides nearby but outside the rafts. Raft-dependent modulation of CaV2.3 could be important in the physiological and pathophysiological processes in which these channels participate, including neuronal excitability, synaptic plasticity, epilepsy, and chronic pain."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00424-014-1605-0"xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Rodriguez-Menchaca A.A."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Meza U."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Sanchez-Armass S."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Leandro D."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Licon Y."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/author | "Romero-Mendez C."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/name | "Pflugers Arch"xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/pages | "1699-1709"xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/title | "Inhibition of CaV2.3 channels by NK1 receptors is sensitive to membrane cholesterol but insensitive to caveolin-1."xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://purl.uniprot.org/core/volume | "467"xsd:string |
| http://purl.uniprot.org/citations/25204428 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25204428 |
| http://purl.uniprot.org/citations/25204428 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25204428 |
| http://purl.uniprot.org/uniprot/#_A6IE24-mappedCitation-25204428 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25204428 |
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| http://purl.uniprot.org/uniprot/#_P25103-mappedCitation-25204428 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25204428 |
| http://purl.uniprot.org/uniprot/#_Q53TQ2-mappedCitation-25204428 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25204428 |
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| http://purl.uniprot.org/uniprot/#_Q4VBL8-mappedCitation-25204428 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25204428 |