| http://purl.uniprot.org/citations/25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25205820 | http://www.w3.org/2000/01/rdf-schema#comment | "Bordetella pertussis toxin (PTx), also known as islet-activating protein, induces insulin secretion by ADP-ribosylation of inhibitory G proteins. PTx-induced insulin secretion may result either from inactivation of Gα(o) proteins or from combined inactivation of Gα(o), Gα(i1), Gα(i2), and Gα(i3) isoforms. However, the specific role of Gα(i2) in pancreatic β-cells still remains unknown. In global (Gα(i2)(-/-)) and β-cell-specific (Gα(i2)(βcko)) gene-targeted Gα(i2) mouse models, we studied glucose homeostasis and islet functions. Insulin secretion experiments and intracellular Ca²⁺ measurements were used to characterize Gα(i2) function in vitro. Gα(i2)(-/-) and Gα(i2)(βcko) mice showed an unexpected metabolic phenotype, i.e., significantly lower plasma insulin levels upon intraperitoneal glucose challenge in Gα(i2)(-/-) and Gα(i2)(βcko) mice, whereas plasma glucose concentrations were unchanged in Gα(i2)(-/-) but significantly increased in Gα(i2)(βcko) mice. These findings indicate a novel albeit unexpected role for Gα(i2) in the expression, turnover, and/or release of insulin from islets. Detection of insulin secretion in isolated islets did not show differences in response to high (16 mM) glucose concentrations between control and β-cell-specific Gα(i2)-deficient mice. In contrast, the two-to threefold increase in insulin secretion evoked by L-arginine or L-ornithine (in the presence of 16 mM glucose) was significantly reduced in islets lacking Gα(i2). In accord with a reduced level of insulin secretion, intracellular calcium concentrations induced by the agonistic amino acid L-arginine did not reach control levels in β-cells. The presented analysis of gene-targeted mice provides novel insights in the role of β-cell Gα(i2) showing that amino acid-induced insulin-release depends on Gα(i2)."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpendo.00337.2014"xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Birnbaumer L."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Schurmann A."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Rath M."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Harteneck C."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Nurnberg B."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Leiss V."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Novakovic A."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Flockerzie K."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/author | "Schonsiegel A."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/name | "Am J Physiol Endocrinol Metab"xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/pages | "E800-12"xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/title | "Insulin secretion stimulated by L-arginine and its metabolite L-ornithine depends on Galpha(i2)."xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://purl.uniprot.org/core/volume | "307"xsd:string |
| http://purl.uniprot.org/citations/25205820 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/25205820 |
| http://purl.uniprot.org/citations/25205820 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/25205820 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWA9-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXC2-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXN0-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |
| http://purl.uniprot.org/uniprot/#_Q922Y6-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |
| http://purl.uniprot.org/uniprot/#_P08752-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |
| http://purl.uniprot.org/uniprot/#_Q8JZT4-mappedCitation-25205820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/25205820 |