| http://purl.uniprot.org/citations/25220281 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25220281 | http://www.w3.org/2000/01/rdf-schema#comment | "Human induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSCs) are a promising source of patient-specific stem cells with great regenerative potential. There has been no report on NEL-like protein 1 (NELL1) gene modification of iPSC-MSCs. The objectives of this study were to genetically modify iPSC-MSCs with NELL1 overexpression for bone tissue engineering, and investigate the osteogenic differentiation of NELL1 gene-modified iPSC-MSCs seeded on Arg-Gly-Asp (RGD)-grafted calcium phosphate cement (CPC) scaffold. Cells were transduced with red fluorescence protein (RFP-iPSC-MSCs) or NELL1 (NELL1-iPSC-MSCs) by a lentiviral vector. Cell proliferation on RGD-grafted CPC scaffold, osteogenic differentiation and bone mineral synthesis were evaluated. RFP-iPSC-MSCs stably expressed high levels of RFP. Both the NELL1 gene and NELL1 protein levels were confirmed higher in NELL1-iPSC-MSCs than in RFP-iPSC-MSCs using RT-PCR and Western blot (P<0.05). Alkaline phosphatase activity was increased by 130% by NELL1 overexpression at 14days (P<0.05), indicating that NELL1 promoted iPSC-MSC osteogenic differentiation. When seeded on RGD-grafted CPC, NELL1-iPSC-MSCs attached and expanded similarly well to RFP-iPSC-MSCs. At 14days, the runt-related transcription factor 2 (RUNX2) gene level of NELL1-iPSC-MSCs was 2.0-fold that of RFP-iPSC-MSCs. The osteocalcin (OC) level of NELL1-iPSC-MSCs was 3.1-fold that of RFP-iPSC-MSCs (P<0.05). The collagen type I alpha 1 (COL1A1) gene level of NELL1-iPSC-MSCs was 1.7-fold that of RFP-iPSC-MSCs at 7days (P<0.05). Mineral synthesis was increased by 81% in NELL1-iPSC-MSCs at 21days. In conclusion, NELL1 overexpression greatly enhanced the osteogenic differentiation and mineral synthesis of iPSC-MSCs on RGD-grafted CPC scaffold for the first time. The novel NELL1-iPSC-MSC seeded RGD-CPC construct is promising for enhancing bone engineering."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.actbio.2014.08.016"xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/author | "Chen W."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/author | "Zhao Z."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/author | "Xu H.H.K."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/date | "2014"xsd:gYear |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/name | "Acta Biomater"xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/pages | "5128-5138"xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/title | "Effect of NELL1 gene overexpression in iPSC-MSCs seeded on calcium phosphate cement."xsd:string |
| http://purl.uniprot.org/citations/25220281 | http://purl.uniprot.org/core/volume | "10"xsd:string |
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